Kao Corporation

Nippon Telegraph and Telephone Corporation

Hitachi, Ltd.

Other

Tokai University Educational System

Cellseed, Inc.

Osaka University

Hokkaido University

Sakai, Hideaki

Hideaki Sakai

Masaru Shimada

Yoshito Jin

Akira Koide

Shin Koike

Kazutoshi Kan

Toyoshige Kobayashi

Minoru Ishibashi

Masao Shimizu

Hideki Mori

Yoshiki Sawa

Tsutomu Nishide

Yukitaka Tanaka

Takatoshi Murase

Shigeru Miyagawa

Takuji Yasukawa

Jun Kohori

Tadashi Hase

Masato Sato

Yasuro Shinohara

Masa-aki Kakimoto

Masahiro Katada

Hideto Takase

Yu Sudo

Furukawa Jun-Ichi

Hiroaki Sugiyama

Yasuhiro Takegawa

Naoki Fujitani

Iichiro Shimomura

Yukiko Imanishi

Norikazu Maeda

By using a graft polymer comprising a dendritic polymer with a styrene skeleton and a hydrophilic polymer grafted to a terminal thereof, a temperature-responsive substrate for cell culture having a temperature-responsive surface for cell culture that allows cells to be cultured with high efficiency and which yet allows cultured cells to be exfoliated in a short period of time and with high efficiency by simply changing the temperature of the substrate surface can be prepared conveniently. If this temperature-responsive substrate for cell culture is used, cells obtained from a variety of tissues can be cultured with high efficiency. If this culture method is utilized, cultured cells can be exfoliated intact in a short amount of time with high efficiency. In addition, by using this graft polymer, a wide range of peptides and proteins can also be separated by simply changing the temperature of a chromatographic carrier. This allows for convenient separation procedure and improves the efficiency of separating operations. What is more, the stereoregularity of the dendritic polymer per se may be utilized to enable separation of solutes based on differences in their molecular structures.

Graft polymer, temperature-responsive substrate for cell culture using the same and production method therefor, as well as liquid chromatographic carrier having the novel graft polymer immomibilized thereon and liquid chromatographic method using the same

The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0&#xb0; C. to 80&#xb0; C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.

Cultured cell sheet, production method thereof, and application method thereof

A culturing apparatus has a culture vessel having a first elastic seal bonded on its upper surface and a culture space formed thereon, and a joint for supplying solution such as a medium to the culture vessel and a second elastic seal having microprojection formed at the lower face. The first elastic seal has a valve for supplying or discharging solution. The second elastic seal is formed with microprojection at the position corresponding to a valve for preventing a spill. The culture vessel and the joint are sucked between the first elastic seal and the second elastic seal, thereby forming an integral seeding device.

Culturing apparatus

A ferroelectric layer () is sandwiched between a lower electrode layer () and an upper electrode (). When a predetermined voltage (DC or pulse) is applied between the lower electrode layer () and the upper electrode () to change the resistance value of the ferroelectric layer () to switch a stable high resistance mode and low resistance mode, a memory operation is obtained. A read can easily be done by reading a current value when a predetermined voltage is applied to the upper electrode ().

Bistable resistance value acquisition device, manufacturing method thereof, metal oxide thin film, and manufacturing method thereof

Disclosed herein is a method for producing fry, which comprises cooking with an oil composition containing at least 15% by weight of diglycerides as a frying oil, wherein the content of nitrogen in at least 5 liters of the oil composition is kept to 0.2% by weight or lower. The method permits repeatedly producing fry good in appearance and flavor without causing coloring of an oil and emission of offensive odor in fry workshops, daily dish shops, eating houses, restaurants, etc. where fry is mass-produced over a long period of time using a diglyceride-containing oil composition having an effect to reduce the accumulation of body fat to prevent obesity.

Production method for fried foods

Disclosed herein is an oil composition comprising the following components (A) and (B):(A) 15 to 70% by weight of diglycerides in which less than 15% by weight of the constitutive fatty acids are (3 type unsaturated fatty acids; and (B) 30 to 85% by weight of a triglyceride in which at least 15% by weight of the constitutive fatty acids are (3 type unsaturated fatty acids. The oil composition is good in flavor, hard to be colored, excellent in hydrolytic stability and resistance to oxidation, good in emulsion stability and excellent in intake balance among fatty acids even under severe conditions that heating is performed at a high temperature for a long period of time, can be widely developed to medicinal preparations and foods and moreover exhibit an excellent inhibitory effect on accumulation of body fat by ingestion of a small amount of diglycerides.

Oil composition

An oil or fat composition containing 15 wt % or more of diglyceride (component (A)) and 70 ppm or more, on the basis of the diglyceride, of an organic carboxylic acid (Component (B)).

Oil or fat composition

The invention provides a fat composition comprising: The fat composition is excellent in oxidation stability, flavor and appearance.

Fat composition

A fat composition is provided containing 55 to less than 95% by weight of diglycerides, in which 55 to less than 93% by weight of the fatty acid groups present are unsaturated fatty acid groups, and a shortening composition containing the fat composition, as well as fried foods containing the fat composition, wherein the shortening is reduced in bubbling during deep frying, and the fried food gives a satisfactory feeling in the mouth and improved crispiness.

Fried food and shortening

Growing community of inventors

Tokyo, Japan

Hideaki Sakai