Vertex Standard Company, Ltd.

Lsi Medience Corporation

Mediford Corporation

Hideaki Kakinuma

Hiroaki Inoue

Takashi Morikawa

Yukiko Shimada

A method of screening for a substance that acts on a cell mass includes producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, adding a test substance to the cell mass, and evaluating an action of the test substance on the cell mass. The cell mass is produced by culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate and producing the cell mass of the primary cancer cells.

Method of screening for a substance that acts on a cell mass

A method of producing a cell mass by three-dimensional culture of primary cancer cells having proliferative ability and properties of handleability, versatility, and high-throughput performance, in which a tumor tissue is used as a starting material, proliferation of cells such as fibroblasts other than cancer cells is inhibited, and the cell mass includes primary cancer cells as a main component. The object is achieved by providing a method of producing a cell mass by three-dimensional culture of primary cancer cells using a tumor tissue, including: a three-dimensional culture step of culturing cells obtained from the tumor tissue in a medium containing a 5% v/v or less extracellular matrix on a substantially low-adhesive cell culture substrate.

Three-dimensional culture of primary cancer cells using tumor tissue

There is provided a microphone apparatus with an adjusting mechanism that prevents wind noise generated at a sound absorbing hole from perceptively inputted in the structure having a microphone built-in at the back of a panel with a sound absorbing hole. A sound box is structured between a sound absorbing hole of a panel and a sound perceptible portion of a microphone, and a movable piece that slidably moves in the sound box is formed. A screw rod is annexed to the movable piece, and a disk screwed with the screw rod is restricted and interposed between a tubular portion, serving as an outer frame of the sound box, and a support portion formed at the lower portion. A part of the disk is exposed to the front surface side from a slit of the panel, and the disk is rotated to move the movable piece, whereby changing a resonant frequency of a ventilation space of the sound box and a ventilation cross.

Microphone apparatus

The present invention provides a radio controlling apparatus that performs a change to a vacant channel rapidly to allow smooth control even if an uncontrollable state due to radio interference suddenly occurs. In a radio controlling transmission apparatus of the radio controlling apparatus, a reception section for searching a vacant channel is provided. When a vacant channel is detected, it is stored in memory of the radio controlling transmission apparatus. A transmission section transmits control signals, a selection code, and vacant channel data, the control signals being output by an operation section using a control signal transmission timer and a selection code transmission timer. Using a reception section, a controlled apparatus of the radio controlling apparatus receives signals. If the vacant channel data and the selection code are received, the controlled apparatus stores them in memory of the controlled apparatus. If control signals are also received, the control signals are sent to a controlled section via a decoder. However, if the selection code is not received by the controlled apparatus for the duration of a selection code holding timer, control abnormality is determined to have occurred. A visible abnormal operation is performed and a reception channel of the controlled apparatus is changed to the vacant channel stored in the memory of the controlled apparatus. In the radio controlling transmission apparatus, a change to the vacant channel is performed and the selection code is transmitted. When the selection code is detected by the controlled apparatus, control of the controlled apparatus is performed using the vacant channel thereafter.

Radio controlling apparatus

An adjustable microphone apparatus including a sound box defined by a peripheral wall surface and adjoining first and second surfaces with an interior as a portion of a sound guide channel for guiding wave forms between a sound absorbing hole and a sound perceptible portion of a microphone in a housing with a movable piece slidably engaged with at least one of the surfaces to move within the interior of the sound box altering the sound wave path through the guide channel upon movement of an accessible portion of an actuator coupled to the movable piece so as to provide a variable sound filter.

Growing community of inventors

Tokyo, Japan

Hideaki Kakinuma