Promega Corporation

Hicham Zegzouti

Said A Goueli

Keith V Wood

Lance P Encell

Thomas A Kirkland

Mary P Hall

Monika G Wood

Poncho Meisenheimer

Brock Binkowski

Thomas Machleidt

Matthew B Robers

James Unch

Melanie Dart

Andrew S Dixon

Nidhi Nath

Marie Schwinn

Subhanjan Mondal

Dileep K Pulukkunat

Juliano Alves

Kevin Hsiao

Brock F Binkowski

Provided herein are compositions and methods for the assembly of a bioluminescent complex from two or more non-luminescent (e.g., substantially non-luminescent) peptide and/or polypeptide units. In particular, bioluminescent activity is conferred upon a non-luminescent polypeptide via structural complementation with another, complementary non-luminescent peptide.

Activation of bioluminescence by structural complementation

Activation of bioluminescene by structural complementation

Provided herein are methods for detecting and quantifying succinate in a sample. Also provided are methods for detecting and quantifying 2-oxoglutarate oxygenase enzyme and/or 2-oxoglutarate oxygenase activity in a sample and methods for screening for modulators of 2-oxoglutarate oxygenase activity.

Bioluminescent succinate detection assay

The invention provides compositions and methods to determine or detect the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP. Methods, kits and compositions for monitoring changes in metabolites such as ADP are disclosed.

ADP detection based luminescent phosphotransferase or ATP hydrolase assay

The disclosure provides compositions and methods to determine or detect ADP or the activity of enzymes, including phosphotransferases such as kinases (e.g., protein, lipid, and sugar kinases) and ATP hydrolases such as ATPases, e.g., HSP90, that employ ATP as a substrate and form ADP as a product by monitoring changes in ADP.

ADP detection based methods using adenylate cyclase and bioluminescence

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Madison, WI, United States of America

Hicham Zegzouti