Soochow University

Hezhong Huang

Yao Lu

Ruiming Jin

Ze Li

The invention provides aIL-6 gene and a detection method for a disease-resistant SNP marker. A cDNA sequence ofIL-6 gene is cloned, as shown in SEQ ID NO: 1. A IL-6 gene gDNA sequence containing an intron of theIL-6 gene is cloned, as shown in SEQ ID NO: 2. A primer for amplifying a disease-resistant SNP locus is designed according to IL-6 gDNA sequence, andIL-6 gene is amplified to obtain an amplification product which is sequenced, and the SNPs loci relevant to virus disease-resistance are found out and the SNP locus is determined according to DNA peak profile. The IL-6 cDNA full-length sequence and IL-6 gDNA full-length sequence are cloned firstly. The SNP locus relevant to virus disease resistance ofIL-6 gene is detected, thereby providing a new method for breeding of

IL-6 gene and detection method of disease-resistant SNP marker thereof

The invention provides aIL-6 gene and a detection method for a disease-resistant SNP marker. A cDNA sequence ofIL-6 gene is cloned, as shown in SEQ ID NO: 1. AIL-6 gene gDNA sequence containing an intron of theIL-6 gene is cloned, as shown in SEQ ID NO: 2. A primer for amplifying a disease-resistant SNP locus is designed according to IL-6 gDNA sequence, andIL-6 gene is amplified to obtain an amplification product which is sequenced, and the SNPs loci relevant to virus disease-resistance are found out and the SNP locus is determined according to DNA peak profile. The IL-6 cDNA full-length sequence and IL-6 gDNA full-length sequence are cloned firstly. The SNP locus relevant to virus disease resistance ofIL-6 gene is detected, thereby providing a new method for breeding of

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Suzhou, China

Hezhong Huang