Centre National De La Recherche Scientifique

Universitè De Montpellier

Université Montpellier 2 Sciences Et Techniques

Centre National De La Recherche Scientifique-cnrs

Universite Montpellier 2

Université Montpellier I

Hervé Cottet

Rachid Matmour

Jean-Philippe Biron

Thomas Le Saux

Michel Martin

Luca Cipelletti

Odile Trambouze

Suzanne Anouti

Jean Philippe Biron

A method for determining the size distribution of a mixture of molecule or particle species including the steps of: injecting a sample of the mixture to be analyzed inside a capillary in which an eluent is flowing; transporting the sample injected along the capillary from an injection section to a detection section thereof, in experimental conditions suitable to generate a Taylor dispersion phenomenon that is measurable at the level of the detection section; generating, by a suitable sensor included in the detection section, a signal characteristic of the Taylor dispersion of the transported sample; processing the detection signal in order to obtain an experimental Taylor signal S(t); and analyzing the experimental Taylor signal &#x15c;(t), wherein the step of analyzing an experimental Taylor signal &#x15c;(t) of a sample of the mixture consists of searching an amplitude distribution P(G) that allows the experimental Taylor signal &#x15c;(t)' to be broken down into a sum of Gaussian functions.

Method for determining the size distribution of a mixture of particles using Taylor dispersion, and associated system

A method for determining the hydrodynamic radius for the constituents of an admixture, includes: (A) by separating capillary electrophoresis, the constituents of the admixture, leaving them within the capillary; (B) at one of the ends of the capillary obtained in this manner, containing, in different zones, the separated constituents, a detectable marker is injected in the region of a detection device which is placed at the side of the other end of the capillary; (C) a pressure difference is induced between the ends of the capillary in order to cause the various constituents separated in step (A) and finally the marker to migrate towards the outlet of the capillary; and (D) by analyzing the Taylor dispersion produced in step (C), the hydrodynamic radius is determined for each of the constituents, based on the detection time of the marker and the elution profile of each of the constituents.

Determination of the hydrodynamic radii of the constituents of an admixture by means of analysis of a taylor dispersion carried out following a separation by means of capillary electrophoresis

A method for separating the constituents of a mixture M by electrophoresis in a single capillary includes (A) separating compounds of the mixture M in a single capillary according to the capillary electrophoresis technique; (B) isolating a fraction F of the compounds thus separated by evacuating part of the compounds having the highest migration speeds from the capillary and/or evacuating part of the compounds having the lowest migration speeds from the capillary; (C) introducing a separating medium MS having a higher migration speed than the compounds of the isolated fraction F into the capillary containing the isolated fraction F, and (D) the compounds contained in fraction F are separated in the new electrophoretic conditions thus obtained.

Bidimensional separation by means of capillary electrophoresis carried out in a single capillary

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Hervé Cottet