Institut National De La Sante Et De La Recherche Medicale

Txcell

Hervé Bastian

Hervé Groux

Arnaud Foussat

Françoise Cottrez

Valérie Brun

Nathalie Belmonte

Brigitte Quattannens

A method for the in vitro production of a cell population P' from a cell population P, the production requiring the presence of at least one factor which is expressed by feeder cells, wherein a) feeder cells proliferate at a temperature T, b) proliferated feeder cells are contacted with the cell population P, c) the cell mixture obtained at step (b) is cultivated at a temperature Twhich is chosen such that the cell population P proliferates and the feeder cells do not proliferate, the at least one factor being expressed by the feeder cells, and d) the cell population P&#x2032; so produced is recovered. Advantageously, the production consists in an expansion, the feeder cells are insect feeder cells and the cell population P to be expanded is a T lymphocyte population, preferably a Trl lymphocyte population.

In vitro production of a cell population using feeder cells

Growing community of inventors

Auribeau sur Siagne, France

Hervé Bastian