United States of America, As Represented by the Secretary of Agriculture

Hernando Duque

Barry Baxt

Michael A Larocco

Foot and out disease virus (FMDV) is worldwide problem. Rapid isolation, serotyping and vaccine matching of FMDV from infected animals is critical to enable the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Current virus isolation protocols use primary cells, known to be susceptible to FMDV, or baby hamster kidney cells (BHK-21) and other cell lines that are not highly sensitive to some strains of FMDV. The &#x3b1;&#x3b2;integrin is a principal receptor for FMDV. We therefore transduced the porcine kidney cell line, LFBK, to stably express both the &#x3b1;and &#x3b2;bovine integrin subunits. The LFBK-&#x3b1;&#x3b2;cell line showed both &#x3b2;expression and enhanced susceptibility to FMDV infection for at least 100 cell passages. LFBK-&#x3b1;&#x3b2;cells are highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophillic strain O/TAW/97 and are thus a sensitive tool for FMDV isolation.

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Greenport, NY, United States of America

Hernando Duque