Massachusetts Institute of Technology

Helicos Biosciences Corporation

Hernan Orgueira

Atanu Roy

Subramanian Marappan

Philip Richard Buzby

Suhaib Siddiqi

Peter H Seeberger

Edyta Olejnik

Peter Schell

The invention provides a family of tethered nucleotide analogs useful in sequencing nucleic acids containing a homopolymer region comprising, for example, two or more base repeats, and to sequencing methods using such tethered nucleotide analogs.

Methods and compositions for sequencing a nucleic acid

Described is a modular, general synthetic strategy for the preparation in solution and on a solid support of heparin, heparin-like glycosaminoglycans, glycosaminoglycans and non-natural analogs of each of them. Additionally, the modular strategy provides the basis for the preparation of combinatorial libraries and parallel libraries of defined glycosaminoglycan oligosaccharides. The defined glycosaminoglycan structures may be used in high-throughput screening experiments to identify carbohydrate sequences that regulate a host of recognition and signal-transduction processes. The determination of specific sequences involved in receptor binding holds great promise for the development of molecular tools which will allow modulation of processes underlying viral entry, angiogenesis, kidney diseases and diseases of the central nervous system. Notably, the present invention enables the automated synthesis of glycosaminoglycans in much the same fashion that peptides and oligonucleotides are currently assembled.

Solid- and solution-phase synthesis of heparin and other glycosaminoglycans

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Cambridge, MA, United States of America

Hernan Orgueira