Forschungszentrum Julich Gmbh

Degussa Aktiengesellschaft

Other

Degussa - Huls Ag

Forschungszentrum Juelich Gmbh

Basf Aktiengesellschaft

Evonik Degussa Gmbh

Kernforschungsanlage Julich Gesellschaft MIT Beschrankter Haftung

Kernforschungsanlage Julich

Pfeifer Langen

Amino Corporation

Rhein Biotech

Kernforschungsanlage Julich Gesellschaft M.b.h.

Hermann Sahm

Lothar Eggeling

Walter Pfefferle

Bettina Mockel

Bettina M&ouml;ckel

Georg Thierbach

Caroline Kreutzer

Petra Ziegler

Petra Peters-Wendisch

Bernhard Eikmanns

Miroslav Patek

Yvonne Tilg

Bernd Eikmanns

Madhavan Nampoothiri K

Mechthild Rieping

Stephan Hans

Bettina Möckel

Anke Weissenborn

Stephanie Bringer-Meyer

Nicole Kennerknecht

Madhavan Nampoothiri

Bert Rehr

Ralf Klasen

Roman Netzer

Christian Riedel

Brigitte Bathe

Achim Marx

Wolfgang Leuchtenberger

Bettina Moeckel

Volker F Wendisch

Ashling McCormack

Harald Seulberger

Kevin Burke

Cliona Stapelton

Roland Freudl

Manfred Paschke

Albert De Graaf

Bruno Kasler

Manfred Kircher

Marina Vrlijc

Stephanie Bringer

Walter Pfefferie

Laurence K Dunican

Bernd Moritz

Bernard Eikmanns

Ruth Siewe

Georg Sprenger

Theodorus Sonke

Robert Faurie

Martin Karutz

Birgit Klassen

Robert Klemps

Siegfried Schoberth

Werner Swyzen

Elisabeth Scheer

Cornelies Petrus Hollenberg

Ingo Matzerath

Wolfgang Klaui

Klaus-Peter Stahmann

Jan Marienhagen

Theo Boddecker

Georg Schmidt

Doris Rittmann

Dieter Reinscheid

Michael Klose

Jochen Meens

Caroline Gerth

A method for producing an L-amino acid comprising: a) cultivating in a medium containing glycerol a recombinant coryneform bacteria which produces the desired L-amino acid and which expresses at least one heterologous polynucleotide of glycerol metabolism, such as glpA, glpB, glpC, glpD, glpE, glpF, glpG, glpK, glpQ, gipT, glpX, gldA, dhaK, dhaL, dhaM, dhaR, fsa, and/or talC, and b) isolating the desired L-amino acid. Preferably, the pathways producing the desired L-amino acid in the coryneform bacteria are amplified.

Method for the fermentative production of L-amino acids with the aid of coryneform bacteria capable of using glycerin as the only carbon source

The invention relates to a method for producing L-valine and to a suitable microorganism. The inventive method is characterized by preferably enhancing the transaminase C activity of a, especially. The organisms so modified have a yield in L-valine which is 35.8% higher than that of non-modified organisms.

Method for the fermentative production of L-valine and suitable microorganism

This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.

Nucleotide sequences of coryneform bacteria coded for proteins participating in L-serine metabolism and method for microbial production of L-serine

The invention relates to nucleotide sequences of coryneform bacteria that encode proteins that are involved in the biosynthesis of L-serine and to a method for producing L-serine. According to the invention, at least 79 amino acids at the C terminus of the wild-type serA sequence are deleted, thereby producing a 3-phosphoglycerate dehydrogenase having a reduced feedback inhibition by L-serine vis-&#xe0;-vis the wild-type sequence.

Nucleotide sequences that encode deregulated phosphoglycerate dehydrogenases of coryneform bacteria and method for producing L-serine

The present invention relates to a process for the microbial production of L-valine in which the dihydroxy acid-synthase (ilvD) activity and/or the ilvD gene expression is intensified in a microorganism. As an alternative or in combination with this, the acetohydroxy acid-synthase (ilvBN) activity and isomeroreductase (ilvC) activity and/or the ilvBNC gene expression are intensified in a microorganism. The process according to the invention preferably makes use of microorganisms in which the activity of at least one enzyme that is involved in a metabolic pathway that reduces the formation of L-valine is weakened or eliminated. Thus, for instance, the process according to the invention preferably makes use of microorganisms having a defect mutation in the threonine dehydratase (ilvA) gene and/or a defect mutation in one or more genes of the pantothenate synthesis.

Method for microbially producing L-valine

A pyruvate carboxylase gene having SEQ ID NO:1 as well as an amino acid sequence having SEQ ID NO:2 expressed by the pyruvate carboxylate gene are disclosed. Also disclosed is a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate carboxylase activity is increased by genetically changing the pyruvate carboxylase gene having SEQ ID NO:1 through expression of a microorganism which produces the corresponding amino acid having SEQ ID NO:2.

Nucleic acid encoding pyruvate carboxylase from coryneform glutamicum

The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

L-Lysine-producing corynebacteria and process for the preparation of L-lysine

The present invention relates to nucleotide sequences of coryneform bacteria, coding for proteins involved in the bio-synthesis of L-serine and to methods for the isolation thereof. The invention further relates to an improved method for the production of L-serine. In addition, the present invention relates to the use of L-serine in the food, animal feed and/or pharmaceutical industries or in human medicine.

Nucleotide sequences coding for proteins involved in the biosynthesis of L-serine, an improved method for the microbial production of L-serine and a genetically modified microorganism suitable therefor

The invention relates to a genetically modified coryneform bacterium, the fadD15 gene of which is amplified, and an isolated polynucleotide which codes for acyl-CoA synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the fadD15 gene in the bacteria and the use of the polynucleotide as a primer or hybridization probe.

FadD15 gene of Corynebacterium glutamicum, encoding an acyl-CoA synthase polypeptide

The invention relates to preferably recombinant DNA derived fromand replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out:

Nucleotide sequences coding for the thrE gene and process for the enzymatic production of L-threonine using coryneform bacteria

The invention relates to isolated nucleotide sequences from Coryneform bacteria which code for the pck gene encoding the enzyme phosphoenol pyruvate carboxykinase (PEP carboxykinase). The invention also relates a process for the fermentative preparation of L-amino acids, in particular L-lysine, L-threonine, and L-glutamate by attenuation of the pck gene.

Nucleotide sequences which code for the pck gene

The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

L-lysine-producing corynebacteria and process for the preparation of lysine

The invention pertains to a process for the microbial production of amino acids. The process involves boosting the export carrier activity and/or export gene expression of a microorganism which produces the desired amino acid. It was found that a single specific gene is responsible for the export of a given amino acid, and on that basis, a process for the microbial production of amino acids, involving the controlled boosting of the export gene expression and/or export carrier activity of a microorganism was developed, which produces the amino acid. The boosted expression or activity of the export carrier resulting from this process increases the secretion rate and thus increases transport of the desired amino acid.

Process for the microbial production of amino acids by boosted activity of export carriers

This invention relates to isolated polynucleotides containing at least one of the polynucleotide sequences selected from the group

Nucleotide sequences coding for the export of branched-chain amino acids, process for the isolation thereof and use thereof

Nucleotide sequences which code for the ACP gene

Growing community of inventors

Jülich, Germany

Hermann Sahm