Dionex Softron Gmbh

Hermann Hochgraeber

Adolf Satzinger

Andreas Unger

Thomas Wachinger

Daniel Buerger

Christoph Hollnagel

Joachim Wiechers

Gervin Ruegenberg

Remco Swart

Robert Springer

Daniel Bürger

Thomas Armin Alexander Eichorn

Burkhard Seyferth

A sample injection method for liquid chromatography is performed with an injection valve having a waste port, two sample loop ports, and two high-pressure ports. One high-pressure port can be connected to a pump and the other high-pressure port can be connected to a chromatography column. A sample loop is connected to one of the sample loop ports on one end and to a pump volume of a sample conveying device on the other end. A section of the sample loop can be separated to facilitate receiving a sample fluid in the sample loop. A control unit controls the injection valve and the sample conveying device. The sample injector allows a sample to be loaded into the sample loop and then pressurized to an operating pressure prior to injecting the sample into the chromatography column. The sample loop may also be isolated from the operating pressure for facilitating depressurization of the loop.

Sample injector for liquid chromatography, particularly for high performance liquid chromatography

A method of introducing a sample into a separation column includes introducing the sample into a trap column, isolating the trap column from ambient atmosphere and pressurizing the trap column to a first pressure while the trap column is isolated from ambient atmosphere, providing a fluid connection between the trap column and the separation column after pressurizing the trap column to the first pressure, supplying the sample from the trap column to the separation column.

Method of introducing a sample into a separation column and corresponding system

A plug unit for connecting capillary tubes includes a plug housing that has an axial borehole, a plug capillary tube that projects through the axial borehole, and a sealing element that surrounds the plug capillary tube. The front end of the plug capillary tube is sealed by an elastic and/or plastic deformation of the sealing element against the capillary tube receptacle opening of a bushing unit. A hollow cylindrical pressure piece is provided that surrounds the sealing element in an axial region facing away from the end surface of the plug capillary tube, and the pressure piece has a rearward end side that faces away from the end surface of the plug capillary tube and that can be loaded by the plug housing with an axial pressure force when the plug unit and bushing unit are connected.

Plug unit and connection system for connecting capillary tubes, especially for high-performance liquid chromatography

A method performed in a liquid chromatography system includes a metering device pushing a sample into a trap column. The metering device sucks in the sample from a sample reservoir, wherein the sucking in the sample from a sample reservoir precedes the step of pushing the sample into the trap column. The liquid chromatography system also includes a trap column and a metering device, wherein the system is adapted to assume a configuration allowing the metering device to push a sample into the trap column and wherein the metering device is adapted to push the sample into the trap column in this configuration, wherein the system is adapted to assume a configuration allowing the sample to be sucked into the system by means of the metering device. Furthermore, the invention relates to a use of the liquid chromatography system for liquid chromatography, in particular of high-pressure liquid chromatography.

Liquid chromatography system, method and use

A method performed in a liquid chromatography system that includes a metering device pushing a sample into a trap column. The metering device sucks in the sample from a sample reservoir, wherein the sucking in the sample from a sample reservoir precedes the step of pushing the sample into the trap column. The liquid chromatography system also includes a trap column and a metering device (), wherein the system () is adapted to assume a configuration allowing the metering device () to push a sample into the trap column () and wherein the metering device () is adapted to push the sample into the trap column () in this configuration, wherein the system () is adapted to assume a configuration allowing the sample to be sucked into the system () by means of the metering device (). Furthermore, the invention relates to a use of the liquid chromatography system () for liquid chromatography, in particular of high pressure liquid chromatography.

The invention relates to a method for setting a gradient delay volume GDV of a liquid chromatography system for a chromatography run in liquid chromatography, in particular a high-performance liquid chromatography system, in which a desired value GDVof a gradient delay volume of the liquid chromatography system is ascertained or predefined and, if the value GDVdeviates from a specific fixed value GDVof a liquid chromatography system, this value GDVis set in a range 0&#x2264;&#x394;GDV=GDV&#x2212;GDV&#x2264;Vof a volume of a volume adjustment device. Furthermore, the invention relates to an automatic sampler for carrying out such a method.

Method for adjusting a gradient delay volume

The invention relates to a sampler for providing a sample for high-performance liquid chromatography, in which a volume of liquid to be taken up into a cylinder can be aspirated by means of a first drive and can be compressed to a high pressure level by means of a second drive independent of the first drive or can be decompressed from this level in a controlled manner.

Growing community of inventors

Offenberg-Neuhausen, Germany

Hermann Hochgraeber