Dsm IP Assets B.v.

Versalis S.p.a.

Herman Jan Pel

Bertus Noordam

Alrik Pieter Los

Johannes Andries Roubos

Bernard Meijrink

Ilse De Lange

Adriana Marina Riemens

Margot Elisabeth Francoise Schooneveld-Bergmans

Noël Nicolaas Maria Elisabeth Van Peij

Liang Wu

Maaike Appeldoorn

Loes Elizabeth Bevers

Lucie Parenicova

Thibaut José Wenzel

Rolf Poldermans

Sylvia Hopper

Noël Nicolass Maria Elisabeth Van Peij

Jan Metske Laan Van Der

Noël Nicolaas Maria Elisabeth Peij Van

Van Noël Nicolaas Maria Elisabeth Peij

De Ilse Lange

The invention relates to a process for producing a fermentation broth by culturing a microorganism.

Process for producing a fermentation broth

The application relates to an enzyme composition, a process for the preparation thereof and the use of the enzyme composition in enzymatic hydrolysis.

Enzyme composition

The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.

Cloning method

The present invention relates to a method based on the use of restriction enzyme digestion and ligation via cleavage sites, thereby to prepare two or more standardized expression cassettes.

The present invention relates to a recombinant host cell for the production of a compound of interest. The invention further relates to a method for the production of such host cell. The invention further relates to the production of a compound of interest. The invention further relates to isolated polynucleotides and vectors and host cells comprising said polynucleotides.

Host cell for the production of a compound of interest

Four new genes, and the enzymes that they encode, are disclosed, which are present inand which are involved in at least five steps in the biosynthetic pathway of vitamin B. The four enzymes are: A: cobyrinic acid a, c-diamide synthase; B: a bifunctional enzyme, which is a cobinamide kinase and a cobinamide phosphate guanyl transferase; C: a cobalamin 5-phosphate synthase; and D: an adenosyl transferase. Genes encoding the four enzymes can be placed inshuttle vectors and used to transformhosts, in order to improve the production of vitamin Bor a precursor thereof on an industrial scale during fermentation.

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Echt, Netherlands

Herman Jan Pel