Lonza Cologne Gmbh

Lonza Cologne Ag

Amaxa Ag

Herbert Müller-Hartmann

Gregor Siebenkotten

Ludger Altrogge

Gudula Riemen

Timo Gleissner

Andreas Heinze

Elke Lorbach

Andreas Wirth

Juliana Helfrich

Meike Weigel

Heike Wessendorf

Corinna Thiel

Michael Habig

Michael Nix

Kirsten Rothmann-Cosic

Rainer Christine

Peter Hoffmann

Günter Kraus

Kirsten Rothmann

Helmut Brosterbus

Timo Gleißner

The invention relates to a method for the application of at least one voltage pulse to at least two containers fitted with electrodes, by means of which at least one voltage pulse is applied to at least one container, while at least one other container is subjected to a preparation or a post-processing. According to the invention the method comprises mutual exchange of the respective positions of the container to which a voltage pulse has already been applied and the further container. Furthermore, the invention relates to a device () for making electrical contact with at least one container fitted with electrodes, with at least one receptacle () upon which at least one container can be set, and with at least one contact appliance () for making contact with the electrodes of the container. According to the invention at least two receptacles () are provided, wherein one receptacle () is located at or inside the contact appliance (), and wherein both receptacles () and/or the contact appliance () can be moved such that after completion of the movement the other receptacle () is located at or inside the contact appliance ().

Method and a device for the electrical treatment of a plurality of containers

The invention relates to a method for applying at least one electrical voltage pulse to at least one reaction space by at least partially discharging at least one charge storing unit for storing electrical charges. According to the invention the method comprises applying at least one voltage pulse to a reaction space by at least partially discharging a second charge storing unit and simultaneously charging or partially discharging a first charge storing. Furthermore, the invention relates to a devicefor applying at least one electrical voltage pulse to at least one reaction space, comprising at least two charge storing units () for storing electrical charges and at least one power supply unit () for charging the charge storing units () and/or at least one discharge device for partially discharging the charge storing units (). According to the invention it is provided that at least two of the charge storing units () form a common storage module which can be discharged at any time.

Method and device for the electrical treatment of reaction spaces

The invention relates to a novel circuit arrangement for electrotransfection or electrofusion, which enables the transportation of DNA and/or other biologically active molecules to the nucleus of higher eukaryotic cells or the fusion of cells, independent of cell division and with reduced cell mortality.

Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher eucaryotic cells using electrical current

The invention concerns a containerwith chamberswhich each comprise at least one pair of electrodes including a firstand a second electrodefor the application of electric voltage for generating an electric field within one chamber. At least two first electrodesof different chambersare conductively coupled and at least one second electrodeof said chamberis separately conductively connectable. The invention further concerns a method for manufacturing said containeras well as a device for electrically contacting at least one of said containers

Container and device for generating electric fields in different chambers

Disclosed is a device for processing biological material which at least comprises a chamber at least closable to the outside and having an inner space for receiving the biological material. The chamber comprises at least one electrode placed in contact with the inner space for generating an electric field. Also disclosed is a method for processing biological material. The biological material is introduced into the inner space above and the electrode can generate an electric field after said biological material is introduced by applying voltage to said electrode and a further electrode in contact with the inner space. The chamber comprises at least one inlet line having at least one opening arranged close to the electrode. The biological material is almost completely rinsed out of the inner space after the electric field is generated, via a solution guided via an inlet line of the chamber along at least one electrode.

Device and method for processing biological material

The invention relates to a containerfor receiving an aqueous solution, which is formed at least partially by an outer limitforming an inner chamberfor receiving the solution, and which comprises at least one area which acts as an electrodewhen an electric voltage is applied and a subsequent discharge occurs, wherein at least one electrodeis made of a conductive synthetic material at least based on a plastic material which is doped with at least one conductive substance. A containerof the above-mentioned kind is created this way, which is simple and economical to produce and also, for example, enables an efficient transfection of living cells by means of electroporation or an effective electrofusion.

Container with at least one electrode

The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce biologically active molecules into cells using an electric current. The inventive buffer solution has a buffering capacity of at least 20 mmol&#xd7;pHand an ionic strength of at least 200 mmol&#xd7;1during a change to the pH value from pH 7 to pH 8 and at a temperature of 25&#xb0; C. The use of a buffer solution of this type allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in the buffer solution.

Buffer solution for electroporation and a method comprising the use of the same

Growing community of inventors

Kö'ln, Germany

Herbert Müller-Hartmann