Kci Licensing, Inc.

Lifecell Corporation

Wright Medical Technology, Inc.

Herbert Daniel Beniker

Hui Xu

Amy K McNulty

Edward S Griffey

Kristine M Kieswetter

David J McQuillan

Patricia A Villanueva

David J Mcquillan

Warren Oliver Haggard

Lawrence E Boerboom

Stephen A Livesey

Amy K Mcnulty

The invention provides acellular tissue matrices made from collagen-containing tissues of animals genetically modified so as to be deficient in the galactose 1,3-galactose epitope and methods of making and using such acellular tissue matrices.

Acellular tissue matrices made from &#x3b1;-1,3-galactose-deficient tissue

Acellular tissue matrices made from alpha-1,3-galactose-deficient tissue

For successful wound healing to occur, the newly formed skin must generate tensile strength through collagen deposition. Measurement of collagen in the granulating wound bed may be predictive of successful wound healing. Existing methods for collagen measurement either require specialized equipment, or do not allow for discrimination of potential interfering molecules. Herein described is an accurate, specific and reliable method to extract and quantify collagen from tissue utilizing high pressure liquid chromatography techniques. The method is sensitive enough to measure the small amounts of collagen found in newly healing wounds.

Method for quantitation of collagen in tissue

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San Antonio, TX, United States of America

Herbert Daniel Beniker