Washington University

Other

Henry V Huang

Douglas E Berg

Dilip K Nag

Sondra Schlesinger

Robin Levis

Charles Moen Rice, Iii

Ping Shen

Cheng Xiong

Suhas H Phadnis

Doulgas E Berg

Barbara Weiss

Manuel Tsiang

A novel transposon useful for sequencing long DNAs is disclosed which comprises a partial sequence of transposon Tn5 with the oligonucleotide primers from phages SP6 and T7 inserted near the opposite ends, respectively, of said transposon Tn5.

DNA transposon Tn5seq1

A small, novel transposon useful for mutagenesis and sequencing DNAs cloned in phage .lambda. is disclosed which comprises a transposon having at each terminus a segment of 19 nucleotides selected from the group consisting of the O-end and I-end sequences of Tn5, at least one restriction enzyme site positioned less that 20 nucleotides distant from each said terminal segment, and a supF amber-suppressor tRNA gene insert.

DNA transposon TN5SUPF in plasmid pBRG1310

Compositions and methods are disclosed for expressing heterologous coding sequences in host cells. The compositions include infectious Sindbis virus RNA molecules containing at least one heterologous coding sequence inserted within the structural region of the Sindbis virus genome. RNA molecules consisting essentially of a Sindbis virus junction region are also provided. Methods utilizing the novel compositions of the present invention to express heterologous coding sequences in transformed host cells are provided. Methods for producing infectious Sindbis virus particles containing infectious Sindbis virus RNA molecules comprising at least one heterologous coding sequence are provided.

Infectious Sindbis virus vectors

Method of DNA sequencing using DNA transposon Tn5seql

DNA transposon TN5SEQ1

The present invention relates to RNA vectors comprising Sindbis virus DI RNA having inserted therein heterologous RNA. The vectors of the present invention provide for the cloning and expression of heterologous RNA in eucaryotic cells as well as the packaging of heterologous RNA into viral particles in the presence of helper virus. Transformed cells and genetically altered alphavirus particles comprising alphavirus DI RNA having inserted therein heterologous RNA are also provided.

Sindbis virus vectors

A method for the simultaneous and rapid quantitative analysis of biological fluids for a plurality of substances, each of which undergoes at least one reaction with a respective cognate compound. Typical substances include hormones, enzymes, and viruses. The method comprises binding each of a plurality of the respective reactive compounds onto a preselected area of a substrate carrier; e.g., to a band or spot on a carrier film and exposing the sensitized carrier to a sample of the biological fluid to permit the unknown substances contained therein to react with their respective cognate compound which is immobilized on the carrier and then removing excess of the sample from the carrier and developing the carrier and measuring the concentration of the reaction products that are at preselected areas of the carrier. The method permits the entire analysis to be automated and a sample such as a blood serum sample to be analyzed for a host of components such as enzymes, hormones, or viruses in a simple, direct and standardized procedure.

Automated method for quantitative analysis of biological fluids

Growing community of inventors

St. Louis, MO, United States of America

Henry V Huang