University of Utah Research Foundation

University of Minnesota

The University of Warwick

Henry S White

Ryan J White

Eric N Ervin

Cynthia J Burrows

Bo Zhang

Ryuji Kawano

Aaron M Fleming

Na An

Gangli Wang

Hakhyun Nam

Richard B Brown

Christopher Ward Macosko

Patrick Robert Unwin

Mark Edward Newton

Joel M Harris

Jun Ho Shim

Julie Macpherson

Shulong Li

Joshua R Wayment

Methods, systems, and compounds for detecting modified nucleic acid bases are disclosed and described. The methods provide for detecting a nucleic acid lesion and can include directing a nucleic acid adduct into a channel, wherein the nucleic acid adduct includes a nucleic acid having a lesion and a current modulating compound coupled to the nucleic acid at the lesion (), and measuring a change in current through the channel in response to the current modulating compound to detect the lesion (). The method can optionally include forming the nucleic acid adduct. Also provided is a method for identifying the number of repeat nucleotides in at least a portion of a nucleic acid strand, a method of assigning a registration marker within a nucleic acid, and a method of obtaining sequence information from a nucleic acid comprising assigning a registration marker on the nucleic acid.

Detection of nucleic acid lesions and adducts using nanopores

A nanopore device includes a membrane having a nanopore extending there through forming a channel from a first side of the membrane to a second side of the membrane. The surface of the channel and first side of the membrane are modified with a hydrophobic coating. A first lipid monolayer is deposited on the first side of the membrane, and a second lipid monolayer is deposited on the second side of the membrane, wherein the hydrophobic coating causes spontaneous generation of a lipid bilayer across the nanopore orifice. Sensing entities, such as a protein ion channel, can be inserted and removed from the bilayer by adjusting transmembrane pressure, and adapter molecules can be electrostatically trapped in the ion channel by applying high transmembrane voltages, while resistance or current flow through the sensing entity can be measured electrically.

Nanopore platforms for ion channel recordings and single molecule detection and analysis

A method of preventing non-specific adsorption of proteins onto a surface can include providing a substrate that has a surface on which surface groups are attached. A solution can be applied to the surface that includes a protective reagent having a terminal functional group exhibiting a dipole moment. A monolayer comprising the protective reagent is assembled on the surface by reacting the protective reagent with the surface groups, thereby creating a protected surface. The protective reagent alone is sufficient to confer to the protected surface an increased resistance to adsorption of proteins.

Surfaces resistant to non-specific protein adsorption and methods of producing the same

Sensor device for ion channel recordings; liquid-liquid measurements and resistive pulse particle counting comprising; at least one sensor element; the element comprising a diamond thin film substrate and a pore which is a nanopore or a micropore included in the substrate. This device may be used in analysis, for instance the device may be used for single molecule detection of an apialyte (e.g. DNA), for the analysis of interactions between a sensor element and an analyte, for the detection of pore forming entities, or for the determination of ion transfer.

Conductivity sensor device comprising diamond film with at least one nanopore or micropore

Provided are fabrication, characterization and application of a nanodisk electrode, a nanopore electrode and a nanopore membrane. These three nanostructures share common fabrication steps. In one embodiment, the fabrication of a disk electrode involves sealing a sharpened internal signal transduction element ('ISTE') into a substrate, followed by polishing of the substrate until a nanometer-sized disk of the ISTE is exposed. The fabrication of a nanopore electrode is accomplished by etching the nanodisk electrode to create a pore in the substrate, with the remaining ISTE comprising the pore base. Complete removal of the ISTE yields a nanopore membrane, in which a conical shaped pore is embedded in a thin membrane of the substrate.

Nanopore electrode, nanopore membrane, methods of preparation and surface modification, and use thereof

Nanopore based ion-selective electrodes and methods of their manufacture as well as methods for their use are disclosed and described. The nanopore based ion-selective electrode can include a pore being present in a solid material and having a nanosize opening in the solid material, a metal conductor disposed inside the pore opposite the opening in the solid material, a reference electrode material contacting said metal conductor and disposed inside the pore, a conductive composition in contact with the reference electrode and disposed in the pore, and an ion-selective membrane. The ion-selective membrane can be configured to isolate the metal conductor, reference electrode material, and conductive composition together within the pore.

Nanopore based ion-selective electrodes

A process is provided to prepare flexible composite polymer fibers by electrochemically forming a coating of a conductive organic polymer on the outer surface of a polymeric fiber.

Electrochemical process for the production of conducting polymer fibers

Growing community of inventors

Salt Lake City, UT, United States of America

Henry S White