Human Genome Sciences, Inc.

Stratagene California

San Diego Antibody

Henry Ji

Craig A Rosen

Jian Ni

Joseph A Sorge

Reiner L Gentz

Paul A Moore

Jing-Shan Hu

Alan L Greener

John C Bauer

Carsten-Peter Carstens

Richard Gibbs

The present invention relates to a non-PCR (polymerase chain reaction) process, particularly a transcription-based amplification method, for amplifying and cloning sequences containing a variable domain sequence such as an immunoglobulin variable domain sequence from the immunological gene repertoire. The present invention comtemplates the expression of antibody library in either in an in vivo expression vector or in an in vitro transcription/translation system. Isolation of a gene coding for a receptor having the ability to bind a preselected ligand and receptors produced by the gene isolated by the method is also contemplated.

Method for cloning variable domain sequences of immunological gene repertoire

Human polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such polypeptides for therapeutic purposes. Antagonists against such polypeptides and their use as a therapeutic are also disclosed. Also disclosed are diagnostic methods for detecting disease which utilize the sequences and polypeptides.

Polynucleotides encoding a protein kinase C inhibitor protein and methods of using these polynucleotides

The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.

Method for ligating nucleic acids and molecular cloning

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San Diego, CA, United States of America

Henry Ji