Hoffmann-la Roche Inc.

Henry J Rosenfeld

Richard A Kaufman

Janine E Sabo

Denise Zaunczkowski

John M Konopka

The invention relates to an improved enzymatic method for determining the concentration of an analyte in a sample body fluid. The improvement comprises a coupled enzyme reaction scheme comprising the in situ generation of reduced nicotinamide coenzyme, with either simultaneous or subsequent re-oxidation of coenzyme by an analyte substrate and substrate specific enzymes. The change in absorbance due to the reoxidation of coenzyme is proportional to the concentration of analyte in the reaction mixture.

In situ nicotinamide coenzyme generating system for enzyme based

An enzymatic assay assemblage for ethanol determination is provided. A diamino compound acts as a buffer and a trapping agent for a product of the enzymatic reaction. Long stability of the assay composition is achieved.

Enzymatic Reagents for ethanol assay containing diamino compounds

An enzymatic assay composition for ethanol determination is provided. A compound of the formula

Enzymatic composition for ethanol assay

A reagent for determining quantitatively the amount of phosphorous in bodily fluids which comprises a surface active agent, a strong acid, ammonium molybdate, a heavy metal chelating compound, and anti-oxidant, and deionized water is described. A method of using such a reagent is also described.

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Florham Park, NJ, United States of America

Henry J Rosenfeld