Bio-rad Laboratories, Inc.

Henry J Jeong

Nathan Lewin

Judith I Blakemore

Vito J Mangiardi

This invention provides a two-site immunoassay method for a ligand utilizing a single incubation. The multivalent ligand, labeled receptor for the ligand and unlabeled receptor for the ligand covalently bound to a solid-phase support are incubated as a substantially stable suspension to produce a solid and liquid phase. The solid and liquid phases are separated from each other and the labeled receptor in either phase is quantitated. The method may be used to assay for human thyroid stimulating hormone using purified, radioactively labeled antibodies and unlabeled antibodies covalently bound to hydrolyzed polyacrylamide particles.

Single-incubation two-site immunoassay

Improved competitive binding serum folate assay in which serum folate bound by folate-selective protein is separated from free serum folate by removal with an anion exchanger, preferably intermediate base anion exchange resin. Preferable are resins characterized by having an aliphatic lattice, particularly, a polyalkyleneamine lattice having tertiary and quaternary amine groups. The resin is advantageously added for the separation function in tablet form.

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Sacramento, CA, United States of America

Henry J Jeong