Ortho Diagnostic Systems Inc.

Ortho Diagnostics, Inc.

Ortho Diagnostic, Inc.

Henry A Graham, Jr

Johnna B Hawk

Rosemary K Chachowski

Chi-Deu Chang

Ernest G Schutt

Houston G Brooks, Jr

Utpal R Chakraborty

David J Olekna

Jennifer A Nasser

Sharon R Lawler

Lloyd L Hollenbeck, Jr

Albert Venturini

Steven R Savitz

Dennis M Mochnal

Walter J Yarrow, Jr

C Minot Dole

Joseph R Paradis

Diane M Barrett

Diane B Kebles

Peter Lisi

A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.

Kit containing glutaraldehyde coated colloidal metal particles of a

Method of producing a metal sol reagent containing colloidal metal

Immunoassay test kit

A method is provided for substantially increasing the dynamic range of an immunoassay and forestalling the hook effect observed with very large ligand concentrations in aqueous samples. The method comprises the addition of ligand binding partners which do not have labels associated therewith to compete with labeled ligand binding partners thereby effectively eliminating the presence of the excess ligand in the sample solution. The conversant method for ligand binding partner immunoassays is also provided.

Method for forestalling the hook effect in a multi-ligand immunoassay

Centrifuge tube for separating and washing red blood cells

A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, and wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through the orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.

Multiple particle washing system and method of use

A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, an air vent and, wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated within the inner tube but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.

Particle washing system and method of use

Reagents and methods are described for an immunoassay test of increased sensitivity and decreased complexity employing an immunoglobulin specific for an antigen naturally or artificially placed upon the surface of an indicator particle coupled through the use of a hetero-bifunctional coupling reagent to a second antibody of differing specificity and specific for the antigen to be detected. In a preferred embodiment, a hetero-bifunctional coupling agent couples via a sulfhydryl group, a univalent immunoglobulin specific for the surface antigens on erythrocytes to a second multivalent immunoglobulin through an amide linkage with the latter immunoglobulin wherein said second immunoglobulin is specific for hepatitis-B surface antigen.

Double antibody conjugate

Methods and reagents for determination of Rh.sub.o (D), C, c, E, e, and K antigens in human blood. The reagents are low-protein compositions containing reduced and S-alkylated IgG antibody to the selected antigen maintained at a pH of between about 7.5 and 8.3. These reagents meet or exceed FDA standards for potency and specificity of the respective antisera.

Blood typing tests and reagents

Methods and reagents for determination of Rh (D) antigen in human blood. The reagent is a low-protein composition containing reduced and S-alkylated anti-D IgG maintained at pH of between about 7.5 and 8.3. This reagent meets or exceeds FDA standards for potency of anti-D for the slide test of rapid tube test.

Anti-D test and reagent

Growing community of inventors

Annandale, NJ, United States of America

Henry A Graham, Jr