University of California

Henrik Vibe Scheller

Pamela C Ronald

Laura E Bartley

Dominique Loque

Dawn Chiniquy

Soe Myat Htwe

Andrew Brandon

Joshua L Heazlewood

The present invention provides for a polypeptide capable of dominant suppression of a first naturally occurring IRX10, wherein the polypeptide comprises an amino acid sequence having at least 70% identity as compared to a second naturally occurring IRX10 wherein the polypeptide comprises one or more of the conserved amino acid indicated in FIG.substituted with a different amino acid residue.

Mutant xylan biosynthetic enzymes capable of dominant suppression of xylan biosynthesis

The invention provides methods of engineering plants to modulate hydroxycinnamic acid content. The invention additionally provides compositions and methods comprising such plants.

Modulation of expression of acyltransferases to modify hydroxycinnamic acid content

The invention provides methods for engineering plants to have reduced levels of acetylation by decreasing expression of one or more Cas1L genes. Such plants can be used, e.g., to increase yield for biofuel production.

Method of reducing acetylation in plants to improve biofuel production

The invention provides compositions and methods for inhibiting the expression of the gene XAX1 in grass plants. Plants with inhibited expression of XAX1 have use, e.g., in biofuel production by increasing the amount of soluble sugar that can be extracted from the plant.

Inhibition of a xylosyltransferase to improve saccharification efficiency

The present invention provides for dominant negative mutations ofREDUCING WALL ACETYLATION (RWA).

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The disclosure provides methods of engineering plants to modulate galactan content. Specifically, the disclosure provides methods for engineering a plant to increase the galactan content in a plant tissue by inducing expression of beta-1,4-galactan synthase (GALS), modulated by a heterologous promoter. Further disclosed are the methods of modulating expression level of GALS under the regulation of a transcription factor, as well as overexpression of UDP-galactose epimerse in the same plant tissue. Tissue specific promoters and transcription factors can be used in the methods are also provided.

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Millbrae, CA, United States of America

Henrik Vibe Scheller