Advandx, Inc.

Boston Probes, Inc.

Applied Biosystems, LLC

Dako A/s

Other

Hologic, Inc.

Applera Corporation

Cytotrack Aps

Life Technologies Corporation

Henrik Stender

Jens J Hyldig-Nielsen

Mark J Fiandaca

Susan Rigby

Kenneth M Oliveira

James M Coull

Kyriaki Stefano

Heather P O'Keefe

Kenneth H Peterson

Elizabeth Bergeron

Kenneth Oliveira

Tom Hede Markussen

Donald B Rising

James R Linder

Lars Ulrik Nielsen

Brian B Lentrichia

Frederick G Bargoot

Erin Coffman

Jan Trnovsky

Anders Skærlund Frandsen

Anne Karin Ildor Rasmussen

Menashi Cohenford

Thais T Johansen

Janeen R Shepard

Robert Naumovski

Kaare Lund

Tina Anderson Mollerup

Lisa L Klimas

Marc Fiandaca

Anne K I Rasmussen

This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the detection of one or more species ofyeast. Non-limiting examples of probing nucleobase sequences that can be used for the probes of this invention can be selected from the group consisting of: AGA-GAG-CAG-CAT-GCA (Seq. Id. No. 1), AGA-GAG-CAA-CAT-GCA (Seq. Id. No. 2), ACA-GCA-GAA-GCC-GTG (Seq. Id. No. 3), CAT-AAA-TGG-CTA-CCA-GA (Seq. Id. No. 4), CAT-AAA-TGG-CTA-CCC-AG (Seq. Id. No. 5), ACT-TGG-AGT-CGA-TAG (Seq. Id. No. 6), CCA-AGG-CTT-ATA-CTC-GC (Seq. Id. No. 7), CCC-CTG-AAT-CGG-GAT (Seq. Id. No. 8), GAC-GCC-AAA-GAC-GCC (Seq. Id. No. 9), ATC-GTC-AGA-GGC-TAT-AA (Seq. Id. No. 10), TAG-CCA-GAA-GAA-AGG (Seq. Id. No. 11), CAT-AAA-TGG-CTA-GCC-AG (Seq. Id. No. 12), CTC-CGA-TGT-GAC-TGC-G (Seq. Id. No. 13), TCC-CAG-ACT-GCT-CGG (Seq. Id. No. 14), TCC-AAG-AGG-TCG-AGA (Seq. Id. No. 15), GCC-AAG-CCA-CAA-GGA (Seq. Id. No. 16), GCC-GCC-AAG-CCA-CA (Seq. Id. No. 17), GGA-CTT-GGG-GTT-AG (Seq. Id. No. 18), CCG-GGT-GCA-TTC-CA (Seq. Id. No. 19), ATG-TAG-AAC-GGA-ACT-A (Seq. Id. No. 20), GAT-TCT-CGG-CCC-CAT-G (Seq. Id. No. 21), CTG-GTT-CGC-CAA-AAA-G (Seq. Id. No. 22) and AGT-ACG-CAT-CAG-AAA (Seq. Id. No. 23).

PNA probes, probe sets, methods and kits pertaining to the detection of candida

The present disclosure relates to a method for attaching, detecting and retrieving a single cell on a manipulation medium comprising the steps of: attaching the single cell onto the manipulation medium by applying a carrier liquid; partially drying out the carrier liquid; detecting a property of the single cell by rotating the manipulation medium and scanning a light beam over the manipulation medium; loosening the single cell from the manipulation medium without damaging the single cell by manipulating the single cell with an instrument; and retrieving the single cell from the manipulation medium by aspirating the single cell into a tube, wherein the carrier liquid is dried out to a degree corresponding to that the single cell does not move during the rotation of the manipulation medium and that the single cell can be loosened by the instrument without damaging the single cell. The disclosure further relates to an apparatus for detection and manipulation of one or more object(s).

Method and apparatus for attaching, detecting and retrieving a single cell on a surface

[object Object]

The present invention relates to peptide nucleic acid (PNA) probes, PNA probe sets and methods for the analysis of Gram positive and Gram negative organisms optionally present in a sample. The invention further relates to diagnostic kits comprising such PNA probes.

Molecular gram stain

This invention relates to methods, articles and compositions useful in detecting target substances in an alcoholic preservative solution, and for identifying sensors useful for binding to such targets. The methods allow for the simultaneous performance of sufficient fixation of a sample and binding of a detectable sensor to a target of interest in the sample. In one aspect, a method is provided that comprises contacting a sample suspected of containing a target with a detectable sensor molecule known to bind to such target in an alcoholic preservative solution. The method maybe performed in multiplex form to permit simultaneous analysis of a plurality of targets. Methods for identifying a sensor capable of binding to a desired target in an alcoholic preservative solution are also provided. An alcoholic preservative solution comprising one or more such detectable sensors is also provided. Also provided is a sample comprising a bound sensor provided by such a process. Also provided are kits useful for such methods.

Detection of a target in a preservative solution

Provided herein are methods, kits and compositions to classify fungi. Methods are provided for classification of fungi according to established phenotypes, for example, antimicrobial susceptibility profiles. More specifically, the invention provides methods for the use of PNA probes in diagnostic applications, which will aid in the direction of appropriate therapy against fungi.

Reagents, methods and kits for classification of fungi and direction of anti-fungal therapy

Disclosed is a PNA probe that includes a nucleobase sequence suitable for the detection, identification and/or quantitation of(sensu stricto). In one embodiment, the PNA probe is complementary to a target sequence of 23S rRNA or rDNA from all species of the genus. The invention has a wide range of important uses including detectingin a sample of interest.

The present invention relates to peptide nucleic acid (PNA) probes, PNA probe sets and methods for the analysis of certainspecies optionally present in a sample. The invention further relates to diagnostic kits comprising such PNA probes or PNA probe sets.

This invention relates to the use of genetic probes for detection of the presence of the SCCmec cassette in. In one aspect, the invention allows specific detection and identification of methicillin-resistant(MRSA) in a clinical sample without interference from the presence of other non-methicillin-resistant staphylococci. In another aspect, the invention allows specific detection and identification of methicillin-resistant coagulase negative staphylococci (MRCNS) originating from a clinical sample without interference from the presence of methicillin-resistant

This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the determination of

Disclosed are compositions and methods for analyzing a target sequence in a sample. Generally, the method includes use of at least one pair of probes (Probe A and Probe B). In one embodiment, Probe A hybridizes to wanted and unwanted nucleic acid in the sample and bears a fluorophore and Probe B hybridizes to unwanted nucleic acid in the sample and bears a quencher. Fluorescence signal from Probe A hybridizing to unwanted nucleic acid is quenched by any relatively close hybridization of Probe B hereby increasing the specificity for the presence, amount or absence of the wanted target sequence. In preferred embodiments, the method is referred to as Fluorescence In-Situ Hybridization (FISH). The invention has many useful applications including rapidly detecting a microbial target sequence in a clinical sample.

Method for improved specificity in probe based assays

The present invention relates to methods, kits and compositions suitable for the improved detection, quantitation and analysis of nucleic acid target sequences using probe-based hybridization assays.

Compositions for detecting target sequences

Growing community of inventors

Gentofte, Denmark

Henrik Stender