Novozymes A/s

Novo Nordisk A/s

Novo Zymes North America, Inc

Henrik Bisgard-Frantzen

Allan Svendsen

Torben Vedel Borchert

Carsten Schau Andersen

Helle Outtrup

Soren Kjaerulff

Bjarne Ronfeldt Nielsen

Vibeke Skovgaard Nielsen

Lisbeth Hedegaard

Christel Thea Jorgensen

Sven Pedersen

Torben Halkier

Arne Agerlin Olsen

Eric Allain

Marianne Thellersen

Kevin S Wenger

Pia Van Der Zee

Annette Prento

Lisbeth Anker

The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and exhibits an alteration in at least one of the following properties relative to the parent alpha-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Cadependency and specific activity.

Alpha-amylase mutants

The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises

The invention relates to a novel Termamyl-like alpha-amylase, and Termamyl-like alpha-amylases comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Caconcentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Caconcentrations (relative to the parent).

The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

Alpha-amylase variants

The present invention relates to processes for producing a fermentation product, such as ethanol, from milled starch-containing material comprising (a) saccharifying the milled starch-containing material with a glucoamylase having an amino acid sequence shown in SEQ ID NO: 2, or a glucoamylase being at least 70% identical thereto, at a temperature below the initial gelatinization temperature of said starch-containing material, (b) fermenting using a fermenting organism.

Processes for producing a fermentation product

The present invention relates to a method of constructing a variant of a parent Termamyl-like &#x3b1;-amylase, which variant has &#x3b1;-amylase activity and at least one altered property as compared to the parent &#x3b1;-amylase, comprises

&#x3b1;-amylase mutants

The invention relates to a variant of a parent Fungamyl-like fungal alpha-amylase, which exhibits improved thermal stability at acidic pH suitable for, e.g., starch processes.

Fungamyl-like alpha-amylase variants

The present invention relates to alpha-amylase variants having mutations corresponding to one or more of the following mutations A181E,D,Q,N,V; I201W,F,L; Q264R,D,K,E; Y290R,E,K; and S334A,V,T,L,I,D (using SEQ ID No. 2 for numbering).

The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

Growing community of inventors

Bagsvaerd, Denmark

Henrik Bisgard-Frantzen