Novo Nordisk A/s

Novid Nordisk

Henrik Bisgangrd-Frantzen

Helle Outtrup

Allan Svendsen

Martin Schulein

Claus Dambmann

Arne Agerlin Olsen

Torben Vedel Borchert

Pia Van Der Zee

Per Linaa Jorgensen

Michael Dolberg Rasmussen

Mads E Bjoernvad

Marianne Thellersen

Peter Rahbek Stergaard

Noriko Tsutsumi

The invention relates to a variant of a parent Termamyl-like .alpha.-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to said parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca.sup.2+ dependency and specific activity.

.alpha.-amylase mutants

The present invention relates to variants of a parent .alpha.-amylase, which parent .alpha.-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an .alpha.-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an .alpha.-amylase having one of these amino acid sequences; in which variant:

Amylase variants

The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.

Starch conversion process

The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprises

This invention relates to Alkalophilic Bacillus sp. AC13 microorganisms and cultures, and mutants and variants thereof, and to enzymes obtainable from the microorganism.

Alkalophilic Bacillus sp. AC13 and protease, xylanase, cellulase

The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprising i) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like .alpha.-amylase variant for the property in question.

The present invention relates to novel microorganisms, novel enzymes obtainable herefrom, and to a method of producing the novel enzymes. More specifically, the invention relates to novel enzymes obtainable from strains of the novel alkalophilic species Bacillus sp. AC13. Moreover, the invention relates to a method for producing the enzymes of the invention, and to the use of the enzymes in detergents or in the paper pulp industry.

An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.

DNA constructs and methods of producing cellulytic enzymes

An .alpha.-amylase characterized by having a specific activity at least 25% higher than the specific activity of Termamyl.RTM. at a temperature in the range of 25.degree. C. to 55.degree. C. and at a pH value in the range of pH 8 to pH 10.

Alkaline bacillus amylase

A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.

Alkaline bacilus amylase

The present invention is directed to isolated nucleic acid constructs comprising a nucleic acid sequence encoding xylanolytic enzymes derived from a strain of Bacillus agaradherens, recombinant vectors and host cells comprising such constructs, and methods for obtaining xylanolytic enzymes.

Growing community of inventors

Lyngby, Denmark

Henrik Bisgangrd-Frantzen