Novozymes A/s

Novo Nordisk A/s

Novozymes Ais

Novozymes A/s Krogshoejvej

Henrik Bisg&aring;rd-Frantzen

Allan Svendsen

Torben Vedel Borchert

Carsten Schau Andersen

Helle Outtrup

Claus Dambmann

Noriko Tsutsumi

Arne Agerlin Olsen

Mads E Bjoernvad

Martin Sch&uuml;lein

Bjarne Ronfeldt Nielsen

Vibeke Skovgaard Nielsen

Per Linaa Jorgensen

Lisbeth Hedegaard Hoeck

Christel Thea J&oslash;rgensen

S&oslash;ren Kjaerulff

The present invention relates to isolated nucleic acid sequences encoding polypeptides having alpha-amylase activity &lsqb;E.C. 3.2.1.1&rsqb;, which may be derived from Bacillus. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.

Nucleic acid sequences encoding alkaline alpha-amylases

The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.

Starch conversion process

&agr;-amylase mutants

The invention relates to a variant of a parent Termamyl-like a-amylase, which variant has a-amylase activity and exhibits an alteration in at least one of the following properties relative to parent a-amylase: substrate specificity, substrate binding, substrate cleavage pattern, thermal stability, pH/activity profile, pH/stability profile, stability towards oxidation, Ca dependency and specific activity.

The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase.

Alpha-amylase variants

Amylase variants

An isolated nucleic acid constructs encoding cellulytic enzymes derived from a strain of,recombinant vectors and host cells comprising such constructs, and methods for obtaining cellulytic enzymes.

DNA constructs and methods of producing cellulytic enzymes

The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.

Starch debranching enzymes

The invention relates to a variant of a parent Termamyl-like &agr;-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased thermostability at acidic pH and/or at low Ca,concentrations (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased thermostability at acidic pH and/or at low Ca,concentrations (relative to the parent).

Growing community of inventors

Bagsvaerd, Denmark

Henrik Bisgård-Frantzen

Growing community of inventors

Bagsvaerd, Denmark

Henrik Bisg&aring;rd-Frantzen

Henrik Bisgård-Frantzen