Radiometer Turku Oy

Henri Sund

Harri Takalo

Niko Meltola

Kaj Blomberg

The present invention relates to a background blocking concept for use in time-resolved fluorometry binding assays. More particular, the invention relates to a binding assay and a kit involving the use of the same or similar chelating ligand in lanthanide chelate-labelled analyte-specific biomolecules and as or in a background blocking agent.

Background blockers for binding assays

The present application discloses a luminescent lanthanide chelate comprising one or more chromophoric moieties of the formula (I) or of the formula (III)  wherein R, Rand Reach independently are selected from carbon-containing substituents forming a C&#x2014;O bond with the neighbouring oxygen atom, Rand Reach represent a bond between the chromophoric moiety and other moieties of the chelate, and Lnis a lanthanide ion, as well as the corresponding luminescence lanthanide chelating ligand. The application also discloses a detectable molecule comprising a biospecific binding reactant (such as an antibody) conjugated to the luminescent lanthanide chelate as well as a method of carrying out a biospecific binding assay, the use of such a detectable molecule in a specific bioaffinity based binding assay utilizing time-resolved fluorometric determination of a specific luminescence, and a solid support material conjugated with the luminescent lanthanide chelate.

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Turku, Finland

Henri Sund