Agence Nationale De Valorisation De La Recherche (anvar)

Societe Nationale Elf Aquitaine

Institut National De La Recherche Agronomique (inra)

Henri Heslot

Philippe Fournier

Michel M Guerineau

Claude Gaillardin

Claude Gerbaud

Phillippe Fournier

Michel Aigle

Francois Lacroute

Anne Provost

Hiroshi Fukuhara

Laurence De Louvencourt

Micheline Wesolowski

Bernard Kudla

Hughes Blanc

The present invention relates to ars sequences which are efficacious in Yarrowia lipolytica, as well as to plasmids carrying these sequences. The strains of Y. lipolytica transformed with the said plasmids may be used in fermentation processes enabling proteins of industrial importance to be prepared.

ARS sequence which is efficacious in yarrowia lipolytica

Cloning and expression vector of a heterologous gene in a yeast, characterized in that it comprises at least; all or part of DNA of the plasmide k.sub.1 of Kluyveromyces lactis, a DNA segment incorporating the heterologous gene as well as the sequences providing the expression of said gene in said yeast.

Cloning and expression vector, yeast transformed by such vector and

A hybrid plasmid comprising at least the DNA of a bacterial plasmid, all or part of the DNA of the 2.mu. plasmid of yeast and a segment of DNA including the gene URA.sub.3.sup.+ of yeast. These plasmids are useful as vectors of exogenous DNA in yeasts.

Hybrid plasmids and microorganisms containing them

The cell walls are removed from two distinct auxotropic mutant strains of yeast. The protoplasts thus obtained are fused and the cell walls regenerated. The strains obtained are cultured on a medium where the original auxotrophs cannot develop and the hybrid or recombinant strains are recovered. The strains obtained by the process are useful for preparing yeast proteins.

Process for obtaining hybrid strains of yeasts

Growing community of inventors

Paris, France

Henri Heslot