Euroimmun Medizinische Labordiagnostika Ag

Hospital Clinic De Barcelona

Pls-Design Gmbh

Institut D'investigacions Biomediques August Pi I Sunyer

Henning Seismann

Winfried Stoecker

Winfried Stöcker

Alf Weimann

Thomas Grunwald

Waltraud Suer

Stefanie Rohwer

Ingke Braren

Julia Gruhne

Victor Herbst

Marco Klinge

Mariona Pascal Capdevila

Winfried STÖCKER

Birgit Wichner

A diagnostically useful carrier includes a means for specifically capturing an antibody to a polypeptide from the group including squid MLC1 or squid MLC2 or a variant thereof in a sample from a subject. A method includes the step detecting in a sample from a subject the presence or absence of an antibody to squid MLC1 or squid MLC2. The polypeptide or the carrier or a polypeptide binding specifically to an IgE antibody from the sample of a patient to squid MLC1 or squid MLC2 are useful for the manufacture of a diagnostic kit, preferably for the diagnosis of allergy.

Method and products for the diagnosis of a seafood allergy

The present invention relates to a method for diagnosing an allergy, comprising the steps of bringing into contact of basophils from a whole blood sample of a patient with an allergen in an aqueous solution in the presence of whole blood, with a volume content of whole blood in the aqueous solution of at least 20 percent by volume, under conditions that allow activation of the basophils by the allergen, enrichment of the basophils from step a) and detection of activated basophils, wherein the detection is carried out in that the expression of a marker characteristic of activated basophils is detected by means of chemiluminescence; and a kit comprising a vessel for bringing the basophils into contact with the allergen, wherein the vessel is preferably a microtiter plate, optionally an allergen, an agent for detecting a marker characteristic of activated basophils by means of chemiluminescence, wherein the agent is preferably a ligand against the marker provided with a label capable of chemiluminescence and the ligand more preferably is a monoclonal antibody, optionally an agent for stopping activation of the basophils, an optionally immobilizable ligand that binds to a polypeptide located on the cell surface of basophils independently of its activation state, wherein the ligand is preferably a monoclonal antibody against the polypeptide, and optionally an agent for lysing and/or removing erythrocytes.

Method for detecting basophil activation

The invention relates to nucleic acids encoding a novelvenom protease or fragments thereof, in particular the protease Ves v 4, recombinant vectors comprising such nucleic acids, and host cells containing the recombinant vectors. The invention is further directed to the expression of such nucleic acids to produce a recombinantvenom protease, or recombinant fragments thereof, or synthetic peptides thereof. Such a protease or fragments thereof or synthetic peptides thereof are useful for diagnosis of insect venom allergy and for therapeutic treatment of insect venom allergy.

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Nienwohld, Germany

Henning Seismann