Plant Genetic Systems N.v.

Bayer Bioscience N.v.

Aventis Cropscience, N.v.

Henk Joos

Johan Botterman

Jeroen Van Rie

Herman Van Mellaert

Marnix Peferoen

Bart J Lambert

Herman Hofte

Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively bindingprotoxin or insecticidal part thereof, preferably the toxin thereof.

Prevention of Bt resistance development

Recombinant plant expressing non-competitively binding Bt insecticidal cryatal proteins

Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding,protoxin or insecticidal part thereof, preferably the toxin thereof.

Recombinant plant expressing non-competitively binding BT insecticidal crystal proteins

Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding B. thuringiensis protoxin or insecticidal part thereof, preferably the toxin thereof.

Recombinant plant expressing non-competitively binding Bt insecticidal

Recombinant plant expressing non-competitively binding insecticidal

Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession nos. 5131 and 5132, produce crystal proteins during sporulation that are toxic to Coleoptera. The crystal proteins contain 74 kDa and 129 kDa protoxins, respectively, which can yield 68 and 66 kDa toxins, respectively, as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that codes for its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.

Bacillus thuringiensis insecticidal toxins

A plant cell transformed with all or part of a Bacillus thuringiensis gene coding for either a 130 or 132 kDa protoxin against Lepidoptera, preferably with a part of the gene coding just for the respective 62 or 60 kDa trypsin-digestion product of the protoxin. A plant, regenerated from the transformed cell, is resistant to Lepidoptera.

Growing community of inventors

Aalter, Belgium

Henk Joos