Syngenta Participations Ag

Syngenta Crop Protection Ag

Heng Zhong

Qiudeng Que

Mary-Dell Matchett Chilton

Zhongying Chen

Weining Gu

Myoung Kim

Yaping Jiang

Mark Scott Rose

Ailing Zhou

Yinping Lucy Qin

Erik M Dunder

Yin-Fu Chang

Eric Boudreau

Liang Shi

Shujie Dong

Manuel B Sainz

Wenling Wang

Chengkun He

Sabrina Rouse

Chunyang Fan

Eric Levy

John Ke

Yingping Lucy Qin

Doug Russell

Mark Rose

Yinghui Dan

Erik Martin Dunder

Sabrina Noel Rouse

Mary-Dell Chilton

The present invention relates to methods and compositions for chimeric RNA comprising a guide RNA and bait RNA for modifying a target site in the genome of a cell. Such modifications include integration of a transgene and allelic mutations and modifications of native genes. Also provided are plants comprising a modified nucleic acid sequence compared to the native gene integrated into a targeted genomic site in the plant genome.

Methods and compositions for targeted genomic insertion

The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Targeted insertion sites in the maize genome

The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Methods and compositions for identifying and enriching for cells comprising site specific genomic modifications

The invention provides three novel disarmed strains ofbacteria useful for the transformation of plants. The invention provides three engineeredChry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

strains

The invention provides methods and compositions for detecting and/or quantifying vector backbone in a nucleic acid preparation comprising a polynucleotide of interest using amplification assays that amplify a junction located between the polynucleotide of interest and the vector backbone, under conditions whereby amplification can occur, wherein the junction comprises a recognition site for a nuclease, and detecting the absence of an amplification product, whereby the absence of the amplification product indicates low or no vector backbone and/or quantifying the amount of amplification product to determine the amount of vector backbone in the nucleic acid preparation.

Methods and compositions for determination of vector backbone in a nucleic acid sample

Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.

Transformation of sugar cane

A method for introducing a nucleic acid sequence into the genome of a plant cell and regenerating a transformed plant therefrom, including culturing the plant cell on at least one plant transformation media, the at least one plant transformation media comprising an effective amount of a compound or compounds which may modulate oxidative burst, such as melatonin, 6-hydroxy melatonin (a melatonin analog), N-t-butyl hydroxylamine (NtBH), thiamazole, 1,3 dimethylthiourea, Resveratrol, epicatechin, NADPH, or NADH.

Compositions and methods for modulating oxidative burst in plant transformation

Multiple shoot structures are induced from plant tissues (e.g., shoot apices or axillary buds on an artificial medium) to produce multiple shoot cultures. These multi-shoot cultures are then transformed by known transformation methods. Plants are subsequently regenerated from the transformed cells. Crops that may be efficiently transformed by this method include plants normally recalcitrant to transformation such as sugar beet, sunflower, soybean, cotton, tobacco, tomato, peanuts, melons, watermelon, squash,and pepper.

Methods for stable transformation of plants

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Research Triangle Park, NC, United States of America

Heng Zhong