Stichting Dienst Landbouwkundig Onderzoek

Plant Research International B.v.

Stichting Dienst Landbouwkundig Onderzock

Hendrikus Antonius Cornelis Bakker

Hendrik Jan Bosch

Dionisius Elisabeth Antonius Florack

Gerard Johan Adolph Rouwendal

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTIII that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.

GNTIII expression in plants

The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.

Optimizing glycan processing in plants

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides a plant comprising functional mammalian &#x3b2;1,4-galactosyltransferase and mammalian sialyl transferase for producing complex N-linked glycans that are galactosylated and sialylated, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.

Transgenic plants expressing galactosyltransferase and sialyl transferase

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides a plant comprising a functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.

Plant expressing mammalian &#x3b2;1,4-galactosyltransferase and &#x3b2;1,3-glucuronyltransferase

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTIII that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants. 

GnTIII expression in plants

The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides plants and plant cells comprising of functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, for example mammalian &#x3b2; 1,4-galactosyltransferase, said plants or plant cells additionally comprising at least a second mammalian protein or functional fragment thereof, for example a mammalian antibody, that is normally not present in plants.

Mammalian-type glycosylation in transgenic plants expressing mammalian &#x3b2;1,4-galactosyltransferase

The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus 5 containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.

Growing community of inventors

Hannover, Germany

Hendrikus Antonius Cornelis Bakker