Streck, Inc.

Board of Regents of the University of Nebraska

Stellenbosch University

Northwest University

Hendrik Viljoen

Scott E Whitney

Joel R TerMaat

Matthew R Kreifels

Anne Frederica Grobler

Jason R Gerold

Yuguo Lei

Lizl Cronje

A cell expansion system for culturing and expanding cells in hydrogel tubes is disclosed herein. The cell expansion system includes a cap having an extruder located within the cap and a tubular housing in fluid connection with the cap. The extruder has at least a first and second inlet. The tubular housing includes a cell compatible buffer. The extruder extends from within the cap into the tubular housing. The cell expansion system allows for expanding cells that can significantly reduce the production time and cost, while increasing the production capacity.

Cell expansion system

Disclosed are an apparatus and methods for rapid amplification of nucleic acids. More particularly, the present disclosure relates to an apparatus for mixing a reaction solution during amplification of nucleic acids and to methods for amplifying nucleic acids. Also disclosed are methods for lysing cells in a sample and amplifying nucleic acids.

Expedited PCR with stirring

A thermocycling device and method of operating a thermocycler instrument, the instrument including a sample holder, at least one thermal cycling element, and at least one first and second temperature sensors, for causing the sample holder containing the at least one sample to undergo polymerase chain reaction amplification by repeated cycling between at least a denaturation heating stage and an annealing cooling stage. The first temperature corresponding with the temperature of the sample holder is monitored using the at least one first temperature sensor, and a second temperature corresponding with the temperature external of the sample holder is monitored using the at least one second temperature sensor. Based upon the first temperature and the second temperature, the power that is delivered to the at least one thermal cycling element of the instrument is dynamically controlled.

Rapid thermocycler system for rapid amplification of nucleic acids and related methods

A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are in substantial spatial opposition with an interior space present between opposing modules. One or multiple sample vessels are placed in between the modules such that the vessels are subjected to temperature cycling by the modules. The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling. In embodiments of the invention the sample vessels may be deformable between: a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer.

Thermocycler and sample vessel for rapid amplification of DNA

An improved tube including a closure portion, a strap integrally connected to the closure portion and being configured for defining a living hinge, a body portion having a longitudinal axis and an outer wall generally circumscribing the longitudinal axis, and being integrally and hingedly connected with the closure portion by way of the strap. The body portion including a sample portion being generally elongated along the longitudinal axis and being configured for elastic deformation along a portion of its length, including in a direction that is generally transverse to the longitudinal axis so that at least a portion of the wall structure compressively and resiliently deforms and engages a wall defining an opening in a sample block of a polymerase chain reaction amplification device, and the first outer wall dimension of the sample portion reduces to a smaller second outer wall dimension.

Growing community of inventors

Lincoln, NE, United States of America

Hendrik Viljoen