Dsm N.v.

Gist-Brocades B.v.

Dsm IP Assets B.v.

Gist-Brocades N.v.

Martek Biosciences Corporation

Rymco International Ag

Dsm Anti-Infectives B.v.

Hendrik Louis Bijl

Johannes Martinus Jacobus Visser

Albert Schaap

Johannes Hendrik Wolf

Peter Philip Lankhorst

Daniel Verkoeijen

Kristian Zuur

André De Haan

Sigbertus Godefridus Maria Geraats

Fredericus Johannes Kruyssen

Johannes Hendrick Wolf

Ibrahim ÖZkan

Heinz Niggli

Johannes Martinus Visser

Andre De Haan

Sigbertus Godefridus Geraats

The present invention describes a vegetable oil comprising a polyunsatured fatty acid having at least 20 carbon atoms (LC-PUFA), which oil has

Vegetable oil comprising a polyunsaturated fatty acid having at least 20 carbon atoms

The extraction of a microbial or single cell oil, for example comprising one or more polyunsaturated fatty acids (PUFAs), directly from microbial cells is disclosed which avoids the need for solvents. After fermentation, the microbial cells are pasteurised, washed and the cell walls lysed or disrupted by a mechanical (e.g. homogenisation), physical (boiling or drying), chemical (solvents) or enzymatic (cell wall degrading enzymes) technique. The oil (containing the PUFA) is then separated from the resulting cell wall debris. This is achieved by centrifugation, which results in an oily phase (top layer) that contains the oil which that can be separated from an aqueous phase (containing the cell wall debris). The oil can then be extracted and if necessary the PUFA can be purified or isolated from the oil.

Isolation of microbial oils

The preparation of a proteinaceous substance suitable for use in a foodstuff is described which comprises fungal fells of the order. The cells are grown in a fermentor vessel in a liquid which is mixed during fermentation, after which the RNA content of the fungal cells is reduced to below 4% the fungal cells processed into an edible substance. This substance is then mechanically texturized into edible textured product for inclusion into foodstuffs, for example in the form of chunks as a meat substitute.

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The present invention discloses a microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass deriverd from a pasteurized fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.

Preparation of microbial polyunsaturated fatty acid containing oil from pasteurised biomass

The present invention discloses a microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass derived from a pasteurized fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.

Preparation of microbial polyunsaturated fatty acid containing oil from pasteurized biomass

The present invention discloses a microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability. In addition, a method is described for the recovery of such oil from a microbial biomass derived from a pasteurised fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent.

Microbial polyunsaturated fatty acid containing oil from pasteurised biomass

Production and use of compositions comprising high concentrations of vitamin B12 activity

Sterol extraction with polar solvent to give low sterol, high

The present invention provides a process for the preparation of a composition comprising natural vitamin B12, wherein said process comprises the steps of:

Production and use of compositions comprising high concentrations of

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Vlaardingen, Netherlands

Hendrik Louis Bijl