Carl Zeiss Microscopy Gmbh

Hendrik Herrmann

Johannes Knoblich

Daniel Harangozo

Robert Fessler

Karin Schütze

Raimund Schütze

Reinold Wischnewski

The invention relates to a device for applying a liquid immersion agent into a gap () between a microscope objective () and a sample () to be examined under the microscope, wherein the device has a blasting device () which is designed to introduce the immersion agent laterally into the gap () in a jet (), and the blasting device () is designed to spray the immersion agent into the gap () in the form of a vapor or spray mist jet ().

Apparatus and method for applying a liquid immersion medium into a clearance between a microscope objective and a specimen to be examined under the microscope

The invention relates to a microscope, having: a specimen chamber for a specimen (); at least one microscope objective (); one motor driven objective changer (), which, for the purposes of changing a microscope objective, moves the least one microscope objective () through a change volume (); a control device () controlling the objective changer (); and an apparatus for applying a liquid immersion agent into a gap () between the microscope objective () and a specimen () received in the specimen chamber, said apparatus having a jet device () which is designed to introduce the immersion agent in a jet () laterally into the gap (), the jet device () having an operating position (B) for laterally introducing the immersion agent into the gap (), in which position the jet device () is located in the change volume (), the jet device () is coupled to a drive () for adjusting the location of the jet device (), and the control device () is connected to and configured for control of the drive () in order to control the drive () in preparation for the microscope objective change such that said drive moves the jet device () into a parking position (P), in which the jet device () clears the change volume (), then to control the objective changer () to change the microscope objective and subsequently to actuate the drive () so that said drive brings the jet device () into the operating position (B).

Immersion medium application by means of an injection nozzle

A method for forming an immersion agent film between the sample holder and the objective of a microscope, comprising an automatic immersion module for supplying immersion agent from an immersion agent reservoir to a target position on the sample holder or on the positioning stage, the automatic immersion module having a spraying device with a nozzle connected to a pump for the purpose of generating an immersion agent jet. A dosed immersion agent jet is sprayed via the automatic immersion module onto a target position on the sample holder or a position on the positioning stage, at a defined speed.

Device and method for forming an immersion agent film

A sample container () for receptacle devices for receiving biological objects that may be used for example as collecting devices () in laser microdissection systems is described, in which the sample container () has a coding () with the aid of which the sample holder in the laser microdissection system can be unambiguously identified in order subsequently to be able to allocate correctly biological objects () to be dissected to individual receptacle containers () of the identified receptacle device () or of the identified sample holder (), so as to carry out a fully automated microdissection procedure.

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Bernried, Germany

Hendrik Herrmann