Guardant Health, Inc.

Illumina Corporation

Leland Stanford Junior University

Guardant Helath, Inc.

Helmy A Eltoukhy

Amirali Talasaz

Stefanie Ann Ward Mortimer

Robert C Kain

Darya Chudova

Tarun Kumar Khurana

Poorya Sabounchi

Diana Abdueva

Wenyi Feng

Min-Jui Richard Shen

Bernard Hirschbein

Mostafa Ronaghi

Mark R Pratt

Majid Aghababazadeh

AmirAli Talasaz

Behnam Javanmardi

Xiaohai Liu

Jonathan Mark Boutell

Geoffrey Paul Smith

Abbas A El Gamal

Xiaolin Wu

Randall Smith

Kay Klausing

Andrew Kennedy

Thomas J Joseph

Khaled Salama

Carolyn Tregidgo

Bahram Ghaffarzadeh Kermani

Kevin L Gunderson

John A Moon

Sebastian Bohm

Alexander G Dickinson

Shengrong Lin

Arash Jamshidi

Jian Gong

Francisco Jose Garcia

Neil A Straus

Eric Hans Vermaas

Kenneth M Kuhn

Marcin Sikora

George Sakaldasis

Nnamdi Ihuegbu

Thomas Joseph

Christine Lee

Richard Lanman

Kimberley Banks

Rebecca Nagy

Disclosed herein in are methods and systems for determining genetic variants (e.g., copy number variation) in a polynucleotide sample. A method for determining copy number variations includes tagging double-stranded polynucleotides with duplex tags, sequencing polynucleotides from the sample and estimating total number of polynucleotides mapping to selected genetic loci. The estimate of total number of polynucleotides can involve estimating the number of double-stranded polynucleotides in the original sample for which no sequence reads are generated. This number can be generated using the number of polynucleotides for which reads for both complementary strands are detected and reads for which only one of the two complementary strands is detected.

Methods and systems for detecting genetic variants

The disclosure provides methods for processing nucleic acid populations containing different forms (e.g., RNA and DNA, single-stranded or double-stranded) and/or extents of modification (e.g., cytosine methylation, association with proteins). These methods accommodate multiple forms and/or modifications of nucleic acid in a sample, such that sequence information can be obtained for multiple forms. The methods also preserve the identity of multiple forms or modified states through processing and analysis, such that analysis of sequence can be combined with epigenetic analysis.

Methods and systems for analyzing nucleic acid molecules

The present disclosure provides a method for enriching for multiple genomic regions using a first bait set that selectively hybridizes to a first set of genomic regions of a nucleic acid sample and a second bait set that selectively hybridizes to a second set of genomic regions of the nucleic acid sample. These bait set panels can selectively enrich for one or more nucleosome-associated regions of a genome, said nucleosome-associated regions comprising genomic regions having one or more genomic base positions with differential nucleosomal occupancy, wherein the differential nucleosomal occupancy is characteristic of a cell or tissue type of origin or disease state.

Methods for multi-resolution analysis of cell-free nucleic acids

Display screen or portion thereof with graphical user interface

Disclosed herein are methods, compositions, and devices for use in the early detection of cancer. The methods include preparing cell-free nucleic acid molecules from a subject for sequencing, sequencing a panel of regions in the cell-free nucleic acid molecules, and detecting one or more markers that are indicative of a cancer.

Methods for early detection of cancer

A biosensor is provided including a detection device and a flow cell mounted to the detection device. The detection device has a detector surface with a plurality of reaction sites. The detection device also includes a filter layer. A method is providing including obtaining signal data from an array of light detectors; determining a crosstalk function for each of the light detectors of the array of light detectors; and determining characteristics of analytes of interest based on the signal data using the crosstalk functions.

Biosensors for biological or chemical analysis and systems and methods for same

Growing community of inventors

Woodside, CA, United States of America

Helmy A Eltoukhy