Behringwerke Aktiengesellschaft

Dade Behring Marburg Gmbh

Helmut Peters

Bernhard Auerbach

Klaus Bosslet

Hans-Peter Hauser

Stefan Knapp

Stefan Brust

The present invention is directed to an immunochemical method for qualitatively or quantitatively detecting an analyte in which a first and a second binding component are contacted with the analyte, followed by detection of the binding of the binding components to the analyte. According to the invention, both of the binding components are prepared recombinantly in the same host using different vectors (V1 and V2) for expressing fusion proteins, with the first binding component being expressed as a fusion protein F1 in vector V1 and the second binding component being expressed as a fusion protein F2 in vector V2. The novel method, which can also be employed for simultaneously determining several analytes, is more sensitive and specific than prior known methods.

Immunochemical determination of multivalent analytes

The invention relates to monoclonal antibodies (MAbs) and fragments thereof which bind to defined tumor-associated antigens, principally of small cell lung carcinoma (SCLC), of melanoma, of neuroblastoma and other tumors of neuroectodermal origin, to hybridoma cell lines for the preparation thereof, and to the antigens which can be defined and/or isolated with the aid of these antibodies or antibody fragments. The antibodies, antibody fragments and antigens can be used as diagnostic. aid, active substance or active substance carrier.

Monoclonal antibodies against tumor-associated antigens, a process for

The invention relates to an improved diagnostic method for the immunological determination of NCAM by means of specific binding partners, the one specific binding partner being immobilized on a carrier and the extent of the binding of the analyte to the first specific binding partner being determined by means of a second binding partner which is specific for the analyte and which is labeled either directly or via further binding partners.

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Marburg, Germany

Helmut Peters