Boehringer Mannheim Gmbh

Helmut Jering

Manfred Baier

Sigmar Klose

Rolf Lerch

Dieter Mangold

Gerhard Bienhaus

Udo Becker

Peter Roeschlau

Gunter Pappert

Siegfried Notzel

Rainer Schafer

Ellen Mossner

Johann Berger

The present invention provides a process for the determination of the thyroxine-binding capacity in serum, wherein, for the calibration, there are used at least two different standard solutions which contain substantially the same amount of thyroxine-binding globulin but a differing content of thyroxine. The present invention also provides a standard solution for the determination of the thyroxine-binding capacity containing thyroxine-binding globulin dissolved in a buffer system.

Standard solutions for determination of thyroxine-binding capacity in

A carrier fleece is prepared for use as reagent carrier from which reagents can be dissolved in as assay such as immunological analysis. The carrier fleece contains from 5 to 60% by weight of cellulose-containing fibers, from 40 to 95% by weight of polyester or polyamide polymer fibers or a combination thereof and from 5 to 30% by weight of the fibers of an organic binding agent which has a hydroxyl or an ester group or a combination thereof. In an immunological analysis, the carrier fleece is impregnated with an immunologically active agent such as a beta-galactosidase conjugate and then introduced into a solution of a sample containing an immunologically active substance to be analyzed. The immunologically active agent is eluted into the sample solution and the presence of the immunologically active substance in the sample is determined.

Carrier fleece for use as a reagent carrier from which reagents can be

The present invention provides a process for the quantitative determination of free thyroxine (FT.sub.4) in plasma, serum or whole blood by immunological methods, wherein the sample is incubated for at most 10 minutes with a 10 to 2000 fold insufficiency of labelled anti-T.sub.4 antibodies, referred to the molar amount of total T.sub.4 in the sample, then immediately brought together with immobilized excess T.sub.4, again incubated, the phases separated and the label measured in one of the phases.

Process for the quantative determination of free thyroxine

The present invention provides a process for the preparation of an immuno-reactive, porous carrier material by application of a solution of a first reaction component of an immuno-reaction and of a solution of a second component of an immuno-reaction coprecipitating therewith, incubation of the carrier material impregnated with the solutions for the immuno-precipitation, optional washing and subsequent drying of the impregnated carrier material, wherein a solution of both components of the immuno-reaction is prepared, which solution contains an inhibitor for the immuno-precipitation, the carrier material is impregnated with this solution and then the immuno-precipitation is initiated by removal of the inhibitor or by removal of the inhibiting action.

Process for the preparation of an immune-reactive porous carrier material

This invention describes a process for immuno-chemical quantitative determination of immunologically active substances. The method involves a first incubation of a sample containing the active substance with a labelled binder, which contains an antibody or antibody fragment. After complexing has taken place, solid phase bound active substance identical to the substance being quantitatively determined is added. The solid phase bound substance binds with free binder, and the solid and liquid phases are separated. A second antibody which is specific either to antibody or the solid phase antibody-substance complex is then added to the liquid phase. This second antibody is non cross-reactive with individual complex components. The amount of labelled first antibody bound to second antibody is then determined.

Immune-chemical measurement process for haptens and proteins

The present invention provides a reagent for the optical determination of blood coagulation behavior (Quick test), comprising thromboplastin, a synthetic thrombin substrate and a buffer, characterized by a content of non-proteolytically active thromboplastin.

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Tutzing, Germany

Helmut Jering