Gesellschaft Fur Biotechnologische Forschung Mbh (gbf)

Bayer Aktiengesellschaft

Gesellschaft Fur Biotechnologische Forsching Mbh

Helmut Blocker

Ronald Frank

Hans Fritz

Wolfgang Bruns

John Collins

Friedhelm Maywald

Guido Volckaert

Gisela Kurth

Gudrun Heisterberg-Moutsis

Andreas Meyerhans

Process for the production of primers and template bank therefor

The present invention relates to a peptide having essentially the amino acid sequence of pancreatic secretory trypsin inhibitor (PSTI). The present invention also relates to variants of such peptide wherein one or more of the amino acids in the original sequence are replaced by other amino acids. These peptides show an advantageously modified specificity in their inhibotory action. A method of preparation of the peptides and their pharmaceutical use is also described.

Pancreatic secretory tryspin inhibitor and variants thereof produced by

Process for DNA sequencing using oligonucleotide bank

A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed without being concentrated on a carrier having reversed phase properties and optionally ion exchanger properties, the oligonuleotides without lipophilic protecting group are removed, their protecting group is cleaved off from the adsorbed oligonucleotides, the adsorbed oligonucleotides are washed and eluted from the carrier and then purified in a manner known per se.

Improved process for the purfication of synthetic oligonucleotides

The invention concerns a double-stranded recombinant DNA cloning vector comprising one single insertion site for foreign DNA and one or two labelling sites adjacent or approximate to said insertion site. In the instance of two labelling sites, said sites flank the insertion site. The labelling site(s) can be individually labelled in a fashion that results in the labelling of the 3' end of either strand. The use of the invention in a process affords the ability to individually label only one strand at the 3' end in a simplified method for base sequencing analysis.

Double-stranded vector and process using it

Growing community of inventors

Braunschweig, Germany

Helmut Blocker