Boehringer Mannheim Gmbh

Helmgard Gauhl

Hans Seidel

Albert Roder

Joachim Ziegenhorn

Joachim Siedel

Klaus Beaucamp

Wolfgang Gruber

Herwig Brunner

Rolf Deeg

Hans Mollering

Michael Nelboeck

Herbert Von Der Eltz

Gunter Lang

Axel Schmidt

Ulrich Mayr

Georg Schawohl

Combination composed of 2-methyl-4-isothiazolin-3-one hydrochloride and 5-bromo-5-nitro-1,3-dioxane for the elimination of microbes as well as for the preservation of column materials in aqueous solutions. The biocidal mixture is preferably added at concentrations of 0.001 to 0.3% (w/v).

Preservation of column materials in aqueous solutions

The present invention provides a process for obtaining sarcosine oxidase from micro-organisms by culturing thereof and obtaining the enzyme from the biomass or from the culture broth, wherein a Pseudomonas strain is cultured.

Process for obtaining sarcosine oxidase from microorganisms

The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions. The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it. Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination

Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for

The present invention provides a process for the determination of N-carbamoylsarcosine, wherein a sample solution containing N-carbamoylsarcosine is reacted with N-carbamoylsarcosine-amidohydrolase to give sarcosine, which is then determined. The present invention also provides the enzyme N-carbamoylsarcosine-amidohydrolase, a process for obtaining it and a reagent containing it.

Process and reagent for the determination of N-carbamoylsarcosine with

The present invention provides a process for obtaining cholesterol esterase from micro-organisms by culturing a micro-organism capable of forming cholesterol esterase in an appropriate nutrient medium in the presence of an inductor and obtaining the enzyme from the culture liquid and/or from the cells, therein the inductor used is a compound of the general formula: ##STR1## in which R and R.sub.1 are alkyl or alkoxy radicals containing 14 to 18 carbon atoms and R or R.sub.1 can also be a hydrogen atom and R.sub.2 is an alkylamino radical containing 2 to 8 carbon atoms, an alkyl-trimethylammonium radical containing 3 to 8 carbon atoms, an alkylpyridine radical containing up to 4 carbon atoms in the alkyl moiety or a radical of the general formula --CH.sub.2 --(CHOH).sub.n --CH.sub.2 OH, in which n is a whole number of from 1 to 4.

Process for obtaining cholesterol esterase from microorganisms

The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically. The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

Determination of NAD(P)H or salicylate

For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.

Method and reagent for the determination of glycerin

The present invention provides a process for obtaining cholesterol esterase from micro-organisms of the genus Pseudomonas, wherein Pseudomonas sp. DSM 1280 or DSM 1281 is cultured in an appropriate culture medium in the presence of an inducer and the enzyme is obtained from the culture medium and/or from the cells.

Process for the preparation of cholesterol esterase

The present invention provides a process for obtaining cholesterol esterase from micro-organisms, wherein a micro-organism capable of cholesterol esterase formation is cultured in an appropriate nutrient medium in the presence of lecithin as inducer and the enzyme is obtained from the culture medium and/or from the cells.

Process for obtaining cholesterol esterase from micro-organisms

The present invention provides a process for obtaining cholesterol oxidase, wherein Streptomyces griseofuscus DSM 40191, Streptomyces hygroscopicus 40771, Streptomyces acidomyceticus DSM 40798 and/or Arthrobacter paraffinens DSM 312 are cultured and the enzyme obtained from the culture supernatant and/or the cells.

Process for obtaining cholesterol oxidase

The present invention provides a process for obtaining maltose phosphorylase and/or .beta.-phosphoglucomutase from micro-organisms, wherein the starting material used is selected from Lactobacillus brevis DSM 20054, NCIB 8836, 8561 and 8562, Lactobacillus plantarum DSM 20174 and 43, Lactobacillus reuteri DSM 20016, Lactobacillus fermentum DSM 20052, Streptococcus spec. DSM 1118, DSM 119, DSM 1120 and DSM 1121. The present invention also provides a composition and process for determining .alpha.-amylase, wherein maltose phosphorylase and .beta.-phosphoglucomutase obtained by such a process is used, as a crude extract or in an enriched form, optionally with the addition of .alpha.-glucose-1,6-diphosphate and of divalent manganese ions.

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Tutzing, Germany

Helmgard Gauhl