Novo Nordisk A/s

Helle Fabricius W&ouml;ldike

Sven Hastrup

Finn C Wiberg

Lars S&oslash;egaard Nielsen

Disclosed is a method for high efficiency release of recombinant proteins in eukaryotic cells and more specifically, for enhancing the secretion of Factor VII by co-expression of kex2 endoprotease with FVII in cells of mammalian origin.

Method for the production of factor VII

The invention relates to a method of producing an extracellular protein in a bacterium provided with an inner and an outer cell membrane, the method comprising: (a) providing a recombinant vector including a DNA construct comprising a DNA sequence encoding the prepropeptide or part of the prepropeptide of a bacterial extracellular protease selected from the group consisting of,protease I, Bacillus metalloproteases and Bacillus serine proteases preceding and operably connected to a DNA sequence encoding a desired protein, (b) transforming cells of a microorganism provided with an inner and outer cell membrane with the recombinant vector of step (a), (c) culturing the transformed cells of step (b) under conditions permitting expression of said DNA insert and leakage of the bacterial extracellular protease propeptide fused to the desired protein into the culture medium, and (d) recovering the resulting protein from the medium.

Growing community of inventors

Lynge, Denmark

Helle Fabricius Wöldike

Growing community of inventors

Lynge, Denmark

Helle Fabricius W&ouml;ldike

Helle Fabricius Wöldike