Valtion Teknillinen Tutkimuskeskus

Heli Sirén

Hans Söderlund

Kari Kataja

Ari Hokkanen

Reetta Satokari

Ingmar Stuns

Rauno Virtanen

Kai Kolari

Timo Särme

Stella Rovio

Jan Rautio

A microfluidic chip device (MCD) and its use for performing miniaturized assays on magnetic microbeads (MMs) are described. The MCD is particularly useful for carrying out miniaturized transcript analysis by aiding affinity capturing (TRAC) assays, including PCR. The MCD comprises at least one reaction chamber with sealable liquid connections and at least one fluidic pillar filter in each chamber. The fluidic pillar filter comprises rods with spacings allowing MMs to pass. The sealable liquid connections feed liquid to the reaction chamber, wherein air bubbles are removed. The liquid stream contacts the MMs, which are manipulated with a magnetic rod. The liquid connections enable trapping of the MMs behind the pillar filters or in the channel, while the liquid is changed.

Microfluidic chip devices and their use

Disclosed is a method and apparatus by which a sample can be taken from a process and be analyzed on-line by means of a capillary electrophoresis apparatus. On the feed side of the capillary electrophoresis unit () the sample is continuously collected via a duct () from the process into the sample feeding system and fed into the capillary ducts () through the same duct as is the feed solution, and all the solutions () both on the feed side and on the exit end of the capillary electrophoresis unit () are pumped through the flow ducts, and the liquids flown through the capillary are removed on the exit end of the capillary electrophoresis unit () by means of a liquid treatment system.

Method for analyzing a sample from a process with on-line capillary electrophoresis apparatus and capillary electrophoresis apparatus

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Espoo, Finland

Heli Sirén