Qiagen Gmbh

Other

Becton Dickinson and Company Limited

Max-planck-gesellschaft Zur F&#xf6;rderung Der Wissenschaften E. V.

Helge Bastian

Simone Gauch

Ralf Wyrich

Metin Colpan

Frank A Augello

Petra Feuser

Lynne Rainen

Uwe Oelm&uuml;ller

Matthew Walenciak

Uwe Oelmüller

Markus Sprenger-Haussels

Christoph Erbacher

Thomas Manz

Oliver Müller

Stefanie Vollert

Manfred Roord

Uwe Orth

Radu Anghel

Susanne Ullmann

Uwe Oelmuller

Oliver Mueller

The present invention involves a process for the isolation of nucleic acids on surfaces by means of at least the following steps: charging of a surface from a given direction with nucleic acids; immobilization of the nucleic acids on the surface; release of the immobilized nucleic acids from the surface; and removal of the released nucleic acids essentially in the direction of charging. Preferably the loading takes place from the top.

Method for isolating and purifying nucleic acids on surfaces

The present invention relates to a method of stabilizing and/or isolating nucleic acids, wherein a biological sample containing nucleic acids is contacted with a cationic compound. The invention also relates to said cationic compound per se and to the use of said cationic compound in stabilizing and/or isolating nucleic acids. Furthermore, the invention relates to pharmaceutical compositions, diagnostic compositions, and to compositions used in research, which include cationic compounds or a complex being formed upon contact of said cationic compound with a nucleic acid.

Method of stabilizing and/or isolating nucleic acids

The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.

Process for the separation of double-stranded/single-stranded nucleic acid structures

The invention relates to a method for the stabilization, purification or/and isolation of nucleic acids from biological materials, in particular stool samples which may contain contaminations and interfering substances. Furthermore, a reagent kit suitable for carrying out the method of the invention is described.

Method for isolating DNA from biological materials

A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes at least one gene induction blocking agent in an amount effective to stabilize and inhibit gene induction. The gene induction blocking agent is able to stabilize nucleic acids in the biological sample at the point of collection to block ex vivo gene induction in the sample when stored at room temperature. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, organic solvents, organic reducing reagents, and mixtures thereof. The biological sample is collected directly from the animal and immediately mixed with the gene induction blocking agent without any intermediate processing or handling.

Method and device for collecting and stabilizing a biological sample

A collection container and method for collecting a predetermined volume of a biological sample, and particularly a whole blood sample, includes an effective amount of at least one stabilizing agent. The stabilizing agent is able to stabilize nucleic acids in the biological sample at the point of collection to prevent enzymatic degradation of the nucleic acids. The stabilizing agents include cationic compounds, detergents, particularly cationic detergents, chaotropic salts, ribonuclease inhibitors, chelating agents, and mixtures thereof.

A method for the size reduction of high-molecular structures, in particular high-molecular nucleic acid structures, in samples wherein the high-molecular structures to be size-reduced are passed through a means provided with at least one porous layer the pore size of which decreases in the direction of the passage of the structures to be size-reduced through the porous layer.

Method for the size reduction of high-molecular structures

The use of multi-well blocks with more than 10 wells is suggested for breaking down biological samples in a ball mill, wherein the process which is to be carried out provides that the biological sample, disposed in a multi-well block with more than 10 wells, is broken down in a ball mill and that following this, proteins and/or nucleic acids are isolated. With the ball mill necessary for this task, with milling containers fixed thereto in a holder (,), the milling containers are designed as uniform well blocks (,) and can be fixed in a form-locking manner into the adapted, twist-preventing holder (,).

Process and apparatus for breaking down biological material

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Mettmann, Germany

Helge Bastian