Evolva Sa

Fluxome Sciences A/s

Helga David

Jochen Förster

Michael Katz

Hans Peter Schmidt

Malin Sendelius

Thomas Durhuus

Thomas Thomasen Durhuus

Sara Peterson Bjorn

Sara Peterson Bjørn

Jochen Foerster

Sara Petersen Bjoern

Jochen FÖRSTER

Sara Petersen BJØRN

A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4 -hydroxylase enzyme (C4H), the ratio K(PAL)/K(C4H) is at least 2:1.

Metabolically engineered cells for the production of pinosylvin

A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4-hydroxylase enzyme (C4H), the ratio K(PAL)/K(C4H) is at least 2:1.

Growing community of inventors

Copenhagen, Denmark

Helga David