Biogen Idec Ma Inc.

Biogen Ma Inc.

Helena Yusuf-Makagiansar

Angela Xiaoying Wang

Valerie Liu Tsang

Paul Chen

Nels Eric Pederson

Thomas Ryll

Anurag Khetan

Yao-ming Huang

Jana Dolnikova

The present invention pertains to methods of increasing the efficiency of producing a bioproduct. In some embodiments, the method increases the quantity of a bioproduct produced, or decreases bioproduct production time, in a bioreactor cell culture producing the bioproduct, the method comprising, (a) intermittently or continuously analyzing the concentration of one or more nutrients in the bioreactor cell culture; and (b) adding to the bioreactor cell culture additional nutrient media when the concentration of the one or more nutrients is lower than a target value.

Nutrient monitoring and feedback control for increased bioproduct production

The present invention relates to methods of reducing substitution of amino acids during the production of polypeptides of interest in mammalian cells. By varying the concentration of amino acids in the culture medium, heterogeneity of the polypeptide's amino acid sequence is decreased, leading to higher yields of functional protein.

Method of supplementing culture media to prevent undesirable amino acid substitutions

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San Diego, CA, United States of America

Helena Yusuf-Makagiansar