Cytiva Bioprocess R&d Ab

Cytiva Sweden Ab

Ge Healthcare Bio-sciences Ab

Ge Healthcare Bioprocess R&d Ab

Helena Skoglar

Jean-Luc Maloisel

Bjorn Markus Olovsson

Hanno Ehring

Martin Hall

Lars Mattsson

Karol Lacki

Key Hyckenberg

Roger Lundqvist

Bjorn Noren

Gunnar Malmquist

Mikael Berg

Anna Akerblom

Karolina Busson

Hans Blom

Mikael Carlsten

Linda Mathiasson

Sara Sendabo

Dan Andersson

Linus Laurin

Erik Estrada

Rebecka Chmielowski

Mikael Johan Helmer Eugen Berg

Lars Henning Ivar Mattsson

Karol Maciej Lacki

Petra BÄNGTSSON

The present invention relates to a method for optimizing a bioprocess purification system comprising a bioreactor configured to provide a harvest comprising a target composition, and a purification process arranged downstream the bioreactor and being configured for purification of the harvest to produce a target product having a desired characteristics. The method comprising: a) detecting () at least one quality attribute indicative of characteristics of the target product in a downstream process, b) identifying () correlations between the at least one quality attribute measured in the downstream process and parameters to control a cell culture process in the bioreactor, and c) controlling () the cell culture process to meet the desired characteristics based on the identified correlations, whereby the target characteristics is within a p re-determined range.

Method in bioprocess purification system

The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

Chromatographic methods for purification of proteins from plasma

The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of plasmaproteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.

Separation matrices for purification of biological particles

The present invention relates to a chromatography system and a method therefor. The chromatography system comprising an inlet port () for receiving a sample, an outlet port () for delivering the sample, a detector (), a column (), and a valve () in fluid communication with the inlet port, the outlet port, the detector, and the column. The valve () comprises a first position () wherein the inlet port is in fluid communication with the outlet port via a first fluid path comprising the detector and the column, wherein the detector is arranged upstream the column. The valve comprises a second position () wherein the inlet port is in fluid communication with the outlet port via a second fluid path comprising the detector and the column, wherein the detector is arranged downstream the column.

Chromatography system and a method therefor

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Uppsala, Sweden

Helena Skoglar