The Secretary of State for Defence

The Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland

Helen Lisa Bullifent

Richard William Titball

Ethel Diane Williamson

Julie Miller

Nicola Jane Walker

Leslie William James Baillie

Helen Claire Flick-Smith

Paula Thomson Holden

Andrew William Topping

Leslie William Baillie

Topping Andrew William

An immunogenic reagent which produces an immune response which is protective against, said reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) ofor variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression from. High yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.

Expression system

An immunogenic reagent which produces an immune response which is protective againstsaid reagent comprising one or more polypeptides which together represent up to three domains of the full length Protective Antigen (PA) ofor variants of these, and at least one of said domains comprises domain 1 or domain 4 of PA or a variant thereof. The polypeptides of the immunogenic reagent as well as full length PA are produced by expression fromHigh yields of polypeptide are obtained using this method. Cells, vectors and nucleic acids used in the method are also described and claimed.

A method of enhancing expression of a desired protein at mucosal effector sites, the method including placing the protein to be expressed under the control of a promoter having SEQ ID NO: 2, SEQ ID NO: 3 or SEQ ID NO: 4, or a fragment or variant of any of these that has promoter activity, and causing expression in mucosal cells. Constructs used in the methods, as well as suitable recombinant gut-colonising microorganisms such as aspp., are also described. Such organisms are useful in the preparation of vaccines.

Recombinant microorganisms

Growing community of inventors

Salisbury, United Kingdom

Helen Lisa Bullifent