Abbott Laboratories Corporation

Helen H Lee

Thomas F Zurek

Curtis J Pepe

Kathleen A Hanley

A David Hofferbert

David W Golde

Jessie W Shih

John D Burczak

Emerson W Chan

Priscilla Swanson

Mark H Buytendorp

John E Stephens

Bryan Peterson

Cynthia Tate

Joseph D Rosenblatt

Kenneth B Idler

Debra L O'Donnell

Joan E Johnson

Timothy J Perko

Cheryl T Motley

Eugene Robertson

Peggy Guidinger

Michelle Edwards

Irvin S Y Chen

The present invention relates generally to a novel human T-cell lymphotropic, or leukemia, virus type II (HTLV-II) isolate designated NRA. HTLV-II was originally isolated from a patient with atypical hairy cell leukemia. Preliminary restriction analysis of this isolate demonstrated that it differs genetically from the prototypical HTLV-II isolate Mo. HTLV-II proviral molecular clones were obtained and the entire nucleotide sequence of the virus ascertained. The claimed invention is particularly directed toward the gp46 and p21 envelope proteins encoded by the env gene. Methods and kits for the detection of HTLV-II antibodies employing these envelope proteins are also described.

Methods for the detection of HTLV-II antibodies employing novel HTLV-II NRA envelope peptides

The present invention provides methods for detecting Chlamydia trachomatis infections using a female urine sample.

Detection of cervical chlamydia trachomatis infection

Methods of differentiation of sera containing antibodies to HTLV-I from sera containing antibodies to HTLV-II are provided, along with peptides for their differentiation. Articles of manufacture containing these peptides are provided which allow for the differentiation of HTLV-I from HTLV-II infected sera.

Differentiation of HTLV-I and HTLV-II using synthetic peptides

A disposable reaction vessel for performing nucleic acid amplification assay. The disposable reaction vessel has a penetrable cap that can be penetrated by an automated pipettor to aspirate a portion of an amplified reaction product. The disposable reaction vessel contains the reagents necessary to perform a nucleic acid amplification assay. A patient specimen is added to the unit dose reagents in the disposable reaction vessel and the penetrable cap is closed. The disposable reaction vessel containing the reaction mixture and the specimen undergoes amplification, typically by placing it in a thermal cycler. After amplification the intact disposable reaction vessel is transferred to an automated analyzer where an automated pipettor penetrates the closure membrane and aspirates a portion of the amplified sample for further processing, without removal of the reaction vessel cap. This avoids the generation of potentially contaminating aerosols or droplets.

Reaction tube with a penetrable membrane to minimize contamination

Method of minimizing contamination in amplification reactions using a

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Lake Forest, IL, United States of America

Helen H Lee