Leica Mikrosysteme Gmbh

Leica Microsystems Nussloch Gmbh

Heinz Plank

Rainer Wogritsch

Siegfried Tanki

Reinhard Lihl

Anton Lang

Stefan Thiem

Paul Wurzinger

Michael Zimmermann

Hubert Goll

Thomas Pfeifer

Andreas Hallady

Cveta Tomova

A sample transfer device () for receiving a sample inside the sample transfer device () and for transferring the sample to a processing or analysing unit includes a connection opening () defining a transfer path () along which the sample is to be transferred from a loading position () of the sample inside the sample transfer device () through the connection opening (), a shutter () configured to block the connection opening () or to unblock the connection opening (), and a shielding member () configured to be arranged between the connection opening () and the loading position () to protect the sample from an incoming gas stream when the shutter () unblocks the connection opening ().

Sample transfer device

A modular specimen holder (') for high pressure freezing and/or X-ray crystallography of a specimen has a specimen holding element (&#x2032;) and an extension element (&#x2032;) connectable with and separable from each other; the specimen holding element (&#x2032;) including a tubule (&#x2032;) adapted to hold the specimen and a base element (&#x2032;) adapted to hold the tubule (&#x2032;), wherein a distance from a bottom of the base element (&#x2032;) to a top of the tubule (&#x2032;) is a first distance (d); the extension element (&#x2032;) being connectable with the base element (&#x2032;), wherein, when the extension element (&#x2032;) and the base element (&#x2032;) are connected with each other, a second distance (d) from a bottom of the extension element (&#x2032;) to the top of the tubule (&#x2032;) is larger than the first distance (d).

Modular specimen holders for high pressure freezing and X-ray crystallography of a specimen

In a device () for rapid pressure-freezing an aqueous sample (), such as a biological specimen, a pressurized cooling medium can be fed into a high-pressure chamber () into which a sample holder () containing a sample () is inserted and which is sealed with a pressure-tight seal, to the location of the sample holder () held therein. The high-pressure chamber () comprises a viewing window structure () with a pressure-tight window (), through which light can be directed from the outside onto the sample () located in the sample holder (). The window () can comprise a transparent window element made of a high-pressure-resistant material, wherein the window element () is held by a pressure- and temperature-resistant window bearing provided in the high-pressure chamber.

Device for the light stimulation and cryopreservation of biological samples

An apparatus for staining sample sections, comprising: a chamber that is adapted for fastening sample sections therein; and a pump that is adapted for pumping liquids through the chamber. The chamber has an inlet and an outlet. The inlet can be connected to at least one reservoir of a staining liquid via inlet lines that are controllable by valves. The inlet lines are formed at least in the region of the associated valves by means of elastic hose lines, and the valves are formed as hose pinch valves. This apparatus minimizes or eliminates contaminations arising from staining processes for thin sections that are in particular prepared for examination in an electron microscope.

Staining apparatus for sample sections

A sample stage for processing a sample in an ion beam etching apparatus has positioning arrangements each having a receiving apparatus and a mask, a sample being mountable in the receiving apparatus with reference to an ion beam and positionable relative to the mask. The sample stage includes a mechanism that enables a switchover between respective positioning arrangements so a selected positioning arrangement is respectively orientable toward the ion beam. The sample in the selected positioning arrangement is exposed to the ion beam while the remaining positioning arrangements face away from the ion beam. The positioning arrangements are arranged in one common vessel. A method for sequential preparation of at least two samples in an ion beam etching unit using the sample stage is also disclosed.

Apparatus and method for sample preparation

An automatic loading apparatus () for specimens () for high-pressure cryosubstitution is disclosed. The automatic loading apparatus () is connected to a high-pressure freezing device (). Specimens () are inserted in a holder (). By means of a slider (), the specimens () and holders () are conveyed to the automatic loading apparatus (). The guidance element () allows exact positioning of the holder () in the clamping element (). The automatic loading apparatus makes possible rapid transfer of the holder () having the specimen () from, for example, an optical microscope to the high-pressure freezing device ().

High-pressure freezing device, automatic apparatus for loading specimens into a high-pressure freezing device, and method for loading a high-pressure freezing device

A transport container () for slides () for immunological labeling of thin tissue sections () is disclosed. The transport container () is embodied in the form of a trough which comprises a peripheral delimiting wall () that is closed off by a base (). There is embodied in the interior of the trough on the delimiting wall () at least one peripheral step () on which the slide rests and is spaced away from the base () of the transport container ().

Transport container for slides for immunological labeling for thin tissue sections

An apparatus and a method for immunological labeling of thin tissue sections are disclosed. The treatment fluid is applied in the form of liquid droplets onto at least one slide, the arrangement of liquid droplets corresponding to the arrangement of the thin tissue sections on the carrier plate. Each slide is retained in a transport container, the transport container being embodied in the form of a trough which comprises a peripheral delimiting wall that is closed off by a base. The base of one transport containers constitutes the cover of a transport container arranged beneath it. The transport containers are stacked in a first and a second station.

Apparatus and method for immunological labeling for thin tissue sections

A container () for tissue preparation for the tissue embedding, having a base body (), that is formed out cylindrical shaped side wall (), a floor () and several bridges (). The bridges () subdivide the container () in several sub-containers (). The side wall () has a plurality of slit shaped openings (), that extend essentially over the total height of the side wall ().

Container and apparatus for tissue processing for tissue embedding

A system for staining and coverslipping specimen slides, having a stainer ( ) and a coverslipper ( ) that are arranged next to one another. A transfer device ( ), which transports racks with specimen slides from the stainer ( ) to the coverslipper ( ), is provided between the stainer ( ) and the coverslipper ( ). Transport is automated so that operation by the user is substantially reduced.

System for staining and coverslipping specimen slides

The invention concerns a unit ( ) for automatic delivery of specimen slides to a coverslipper ( ). The unit ( ) comprises a housing ( ) in which a front panel ( ) is provided. Also provided is a passage ( ) in the housing ( ) that is defined substantially by a first and a second cutout ( and ) in the first and the second side wall ( and ). A transfer position ( ) for racks ( ) is provided in the region of the cutout ( ) in the first side wall ( ), and a transport apparatus ( ) extends out substantially from the transfer position ( ) to beyond the cutout ( ) in the second side wall ( ).

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Wiener Neudorf, Austria

Heinz Plank