Basf Aktiengesellschaft

Universitat Heidelberg

Ruprecht-karls-universitat Heidelberg Kirchhoff-institut Fur Physik

Ruprecht-karls-universitat Heidelberg

Heinz Eipel

Christoph Cremer

Harald Keller

Dieter Horn

Walter Ditter

David Michael Baddeley

Roman Amberger

Josef Eisenlauer

Paul Lemmer

Siegfried Hauer

Stefan Emig

David Baddeley

Cristoph Cremer

A method and apparatus are provided for obtaining a sub-resolution spatial information of a sample labeled with at least one type fluorescent label. The sub-resolution spatial information has localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The method acquires localization image data by employing fluorescence localization microscopy. The acquired localization image data is processed to obtain the localization information about the positions of fluorescent molecules of the at least one type fluorescent label in at least one spatial direction. The step of processing includes determining in each of the detected images of the series the positions of the barycenters of the detected fluorescence emission distributions from the single fluorescent molecules of the one or more fluorescent labels in at least one spatial direction.

Method and an apparatus for localization of single dye molecules in the fluorescent microscopy

A total internal reflection microscope for epi-fluorescence illumination observations includes an objective through which an object to be observed is illuminated by an excitation illumination light at an angle to an observation axis of the microscope. The angle is adjustable to be within the range suitable for a total internal reflection observation. The microscope also has a source of collimated excitation light. An interferometer is arranged in the optical path of the collimated excitation light and is configured to produce an interference pattern. A focusing lens system focuses the interference pattern produced by the interferometer into the back focal plane of the objective. The objective and the focusing lens system image the interference pattern produced by the interferometer into the conjugated image plane of the objective, thereby producing excitation illumination light that modulated spatially in intensity in a plane orthogonal to the observation axis of the microscope.

Total internal reflection interferometer with laterally structured illumination

The invention relates to solid supports for analytical measurement methods which are essentially composed of an inert solid support material on which hydrophilic measurement zones, separated from one another by a hydrophobic coating, where at least 10 measurement points are applied to the support per cm . The invention furthermore relates to a process for producing the supports, and to the use of the supports in diagnostic methods, in research looking for active substances, in combinatorial chemistry, in crop protection, in toxicology or in environmental protection.

Solid supports for analytical measuring processes

The present invention relates to a method of depositing small amounts of liquid on a substrate ( ) by using a pipette tip ( ), which is connected to a flexible delivery line ( ). Liquid is taken up by expanding the volume in the delivery line ( ), and liquid is ejected by exposing the line to an impulse that is transmitted to the liquid contained in it.

Method and device for applying small quantities of liquid

The invention relates to an automatic pipetting apparatus, having a drive for moving a pipetting instrument from a source site to a target site, in which apparatus end stops (,), which can be finely adjusted automatically, for the movements of the pipetting instrument (,) generated by the drive are provided at the source site and/or the target site.

Automatic pipetting apparatus

In a laser-optical arrangement for producing signals by light scattering, obscuration or reflection, from flowing particles and/or groups of particles, which are recorded, as time-dependent voltage/current fluctuations, by means of a photodetector (9) via appropriate imaging optics (7,8,18), assignment to the degree of dispersion of the flowing system is based on a subsequent measurement (10) of the root-mean-square value, and, according to the invention, the sample stream (1) is separated from a transmitter (6,7,18) and a receiver (8,9,10) by an enveloping stream (4), and the transmitter side consists of an individual light-transmitting fiber (7) provided with a means for parallel or focused emergence of a light bundle having a diameter of the order of magnitude of the particles and/or groups of particles.

Growing community of inventors

Bensheim, Germany

Heinz Eipel