Aventis Pharma Deutschland, Gmbh

Sanofi-a Ventis Deutschland Gmbh

Heinrich Decker

Michael Kurz

Eberhard Ehlers

Paul Habermann

Jozsef Aszodi

Werner Aretz

Cordula Hopmann

Sebastian Rissom

Frank Zocher

Dominique Le Beller

Christophe Salagnad

Laszl&oacute; V&eacute;rtesy

Oliver Maneg

Guido Seidel

Reiner Olliger

Claus Lattemann

Frank Rainer Schmidt

The invention relates to the production of carboxy-terminal (C-terminal) amidified peptides with C-terminal amidified lysin, in particular with the biological activity of GLP-1, the chemical and/or biotechnological precursors and intermediate products thereof. The invention also relates to a method for the production and use thereof for producing pharmaceutical products.

Method for producing carboxy-terminal-amidified peptides

The invention relates to a method for the enzymatic elimination of the N-acyl side chain from lipopeptides to form the corresponding nucleus, wherein the lipopeptide is prepared by fermentation, the lipopeptide being bound to the cells of the biomass, and the biomass is removed with the adhering lipopeptide, the biomass with the adhering lipopeptide is resuspended in an aqueous system, a suitable deacylase is added in dissolved or solid form to the suspension of the biomass, and the corresponding nucleus is formed, and the nucleus is optionally isolated and purified, wherein the lipopeptide obtained by fermentation is reacted after the end of the fermentation as cell-bound biomass without further purification directly with a deacylase, whereby the N-acyl chain linked via an amide linkage is eliminated.

Method for the deacylation of lipopeptides

The invention relates to a recombinant DNA molecule which comprises genes for biosynthesizing acarbose and homologous pseudo-oligosaccharides; to oligonucleotide primers for the PCR amplification of the molecule; to proteins which can be obtained by expressing the genes located on a molecule; to vectors and host cells which comprise the above-mentioned DNA molecule; to proteins which are encoded by the DNA molecule; to proteins which are expressed by means of said vectors in said host cells; to processes for preparing acarbose by introducing the characterized genes into appropriate host organisms and/or eliminating these genes from the host organisms; to processes for completing the gene cluster of genes for biosynthesizing acarbose, to processes for isolating analogous gene clusters in organisms other than GLA.O, to processes for mutating promoters of endogenous acarbose biosynthesis genes for the purpose of increasing the yield of acarbose, to the use of GLA.O for preparing acarbose and for preparing mutants of GLA.O which are optimized with regard to the acarbose yield.

Isolation of biosynthesis genes for pseudo-oligosaccharides from Streptomyces glaucescens GLA.O, and their use

Amycomycin, a process for its production and its use as a pharmaceutical

Calcium salts of lipopeptide antibiotics, method for producing same and their use

The invention relates to a recombinant DNA molecule which comprises genes for biosynthesizing acarbose and homologous pseudo-oligosaccharides; to oligonucleotide primers for the PCR amplification of the molecule; to proteins which can be obtained by expressing the genes located on a molecule; to vectors and host cells which comprise the above-mentioned DNA molecule; to proteins which are encoded by the DNA molecule; to proteins which are expressed by means of said vectors in said host cells; to processes for preparing acarbose by introducing the characterized genes into appropriate host organisms and/or eliminating these genes from the host organisms; to processes for completing the gene cluster of genes for biosynthesizing acarbose, to processes for isolating analogous gene clusters in organisms other than,GLA.O, to processes for mutating promoters of endogenous acarbose biosynthesis genes for the purpose of increasing the yield of acarbose, to the use of,GLA.O for preparing acarbose and for preparing mutants of,GLA.O which are optimized with regard to the acarbose yield.

Isolation of the biosynthesis genes for pseudo-oligosaccharides from streptomyces glaucescens GLA.O, and their use

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Bremtal, Germany

Heinrich Decker