Somagenics, Inc.

Integrated Dna Technologies, Inc.

Heini Ilves

Brian H Johnston

Anne Dallas

Qing Ge

Mark Aaron Behlke

Sergei A Kazakov

Roger Louis Kaspar

Alexander V Vlassov

Sumedha Jayasena

Attila A Seyhan

Catharina Casper-Lindley

Wound healing is a complex homeostatic process in which several distinct types coordinate to repair a physical damage. Failure to close wounds contributes to the pathology of conditions like diabetes mellitus, particularly in the elderly. Presented herein are molecules, pharmaceutical compositions, and methods for applying small RNA oligonucleotide technology to wound healing. Small RNA oligonucleotide approaches as disclosed herein provide a therapeutic strategy for improving both basal and pathological wound healing.

Methods and compositions of short small hairpin RNAs and microRNAs for wound healing

Aspects of the present invention include the production and use of chemically modified RNAi agents (e.g., shRNAs) in gene silencing applications. The chemically modified RNAi agents disclosed herein have reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding RNAi agent not having the chemical modification. Compositions containing chemically modified RNAi agents according to aspects of the present invention (including pharmaceutical compositions) and kits containing the same are also provided.

Chemical modification of short small hairpin RNAs for inhibition of gene expression

Methods and compositions of short small hairpin RNAS and microRNAS for wound healing

Disclosed herein are compositions and methods for the processing, amplification and detection of polynucleotides using target-specific oligonucleotides (TSOs). Hybridization of TSOs to target polynucleotides guides target processing into and purification of small target fragments that then can be amplified and detected with high sensitivity and reproducibility. The method is specifically beneficial for highly degraded polynucleotides found in biological samples.

Methods and compositions for detecting polynucleotides and fragments thereof

Methods, compositions, and kits that include small hairpin RNA (shRNA) useful for inhibition of gene expression, such as viral-mediated gene expression, are described.

Short hairpin RNAs for inhibition of gene expression

The invention provides methods, compositions, and kits comprising small interfering RNA (shRNA or siRNA) that are useful for inhibition of viral-mediated gene expression. Small interfering RNAs as described herein can be used in methods of treatment of HCV infection. ShRNA and siRNA constructs targetING the internal ribosome entry site (IRES) sequence of HCV are described.

Growing community of inventors

Santa Cruz, CA, United States of America

Heini Ilves